|
| Status |
Public on Apr 28, 2009 |
| Title |
Normal BC0171N |
| Sample type |
RNA |
| |
|
| Source name |
normal breast tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast
histopathological exam: normal
grade: normal
age in years: 57
race: Malay
|
| Treatment protocol |
n/a
|
| Growth protocol |
tissues were collected from the operation theater and immediately stored in liquid nitrogen prior to total RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNAs were extracted using Trizol reagent and purified through Qiagen purification column according to manufacturer's recommendations
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNAs
|
| |
|
| Hybridization protocol |
Following fragmentation, 35 ug of cRNA were hybridized for 16 hr at 45C on Human U133A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 2500.
|
| Description |
gene expression data from normal breast tissue
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) and GCOS version 2.0 using Affymetrix default analysis settings. The global trimmed mean signal intensity value were set to ensure the scale factors were in the range of 1.000 – 3.000.
|
| |
|
| Submission date |
Apr 27, 2009 |
| Last update date |
Apr 27, 2009 |
| Contact name |
Ivyna PN Bong |
| E-mail(s) |
ivyna@moh.gov.my
|
| Phone |
+603 26162715
|
| Organization name |
Institute for Medical Research
|
| Department |
Cancer Research Centre
|
| Lab |
Genetics
|
| Street address |
Jalan Pahang
|
| City |
Kuala Lumpur |
| State/province |
Kuala Lumpur |
| ZIP/Postal code |
50588 |
| Country |
Malaysia |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE15852 |
Expression data from human breast tumors and their paired normal tissues |
|
