GEO DataSets

(Submitter supplied) The identification of genes regulated by the transcription factor Bip1 during appressorial development. The rice blast fungus Magnaporthe grisea develops melanized appressoria with high turgor pressure that inject the fungus into epidermal host cells. A screen for M. grisea non-pathogenic mutants recovered mutant M763 with reduced penetration efficiency and infectious growth. Complementation analysis identified a gene inactivated in M763 that encodes a novel basic leucine zipper (bZIP) transcription factor named Bip1 (B-ZIP Involved in Pathogenesis1). more...

Organism:

Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL7266

12 Samples

Download data: GPR

(Submitter supplied) M. oryzae 70-15 was treated with multiple bacteria which inhibit 70-15's growth. The bacterial treatments were EA105, a pseudomonad naturally isolated from rice soil, as well as P. fluorescens biocontrol strain CHAO and the cyanide non-producing mutant of CHAO, CHA77.

Organism:

Pyricularia oryzae 70-15; Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL9201

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL9203

24 Samples

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(Submitter supplied) Genes potentially regulated by the Flb4p transcription factor were identified by microarray-based transcriptome analysis of the flb4- mutant and the parental strain 70-15.

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL9203

12 Samples

Download data: TXT

(Submitter supplied) Genes potentially regulated by the Flb3p transcription factor were identified by microarray-based transcriptome analysis of the flb3- mutant and the parental strain 70-15.

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL9203

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platforms:

GPL7271 GPL9203

11 Samples

Download data: TXT

(Submitter supplied) In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in conidia of the tra1- mutant and the parental strain 70-15.

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platform:

GPL7271

3 Samples

Download data: TXT

(Submitter supplied) In order to understand the function of the Tra1p transcription factor we identified potentially Tra1p-regulated genes by comparison of transcript abundance in aerial mycelium of the tra1- mutant and the parental strain 70-15.

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL9203

8 Samples

Download data: TXT

(Submitter supplied) The rice blast fungus Magnaporthe oryzae is a major constraint to the world food security. To study the intracellular mechanism involved in its pathogenic morphogenesis, digital gene expression tag profiling was used to compare a previously generated temperature sensitive mutant, T146, which stopped pathogenic morphogenesis at 33°C, with its related wide type, Guy11. 416 genes were detected as differential expressed genes, 26 of which were specifically expressed in Guy11 but stopped expression in the mutant. more...

Organism:

Pyricularia grisea

Type:

Expression profiling by SAGE

Platform:

GPL15220

2 Samples

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(Submitter supplied) Rice blast is the most threatening disease to cultivated rice worldwide. Magnaporthe oryzae, its causal agent is likely to encounter environmental challenges during invasive growth in the host plants that require shifts in gene expression to establish a compatible interaction. Here, we tested the hypothesis that M. oryzae have similar gene expression patterns in planta, versus during in vitro stresses. more...

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platform:

GPL9203

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array; Genome binding/occupancy profiling by array

Platforms:

GPL9182 GPL9203

19 Samples

Download data: GFF, PAIR, TXT

(Submitter supplied) The Ca2+/calcineurin signaling pathway is a central conduit regulating growth, development, and virulence of fungal pathogens infecting plants and human. We have analyzed global gene expression profiles during Ca2+ treatment in the rice blast fungus, Magnaporthe oryzae. An immunosuppressive drug, FK506, and the knock-out mutant of a transcription factor, MoCRZ1, were included to analyze calcineurin- and/or CRZ1-dependent gene expression, respectively. more...

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platform:

GPL9203

16 Samples

Download data: TXT

(Submitter supplied) CRZ1 (Calcineurin Responsive Zinc finger 1) is a conserved transcription factor that relays Ca2+/calcineurin signals within the cell. To catalog genome wide locations that MoCRZ1 binds to, we have conducted chromatin immunoprecipitation coupled with microarray (ChIP-chip) in the rice blast fungus, Magnaporthe oryzae. Fungal mycelia were treated with 200mM CaCl2 to activate translocation of MoCRZ1 to the nucleus, thereby binding to the promoter regions of downstream genes, and CaCl2 together with 10ug/ml FK506 to block these phenomena. more...

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Genome binding/occupancy profiling by array

Platform:

GPL9182

3 Samples

Download data: GFF, PAIR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Oryza sativa Japonica Group; Pyricularia grisea; Pyricularia oryzae; Oryza sativa

Type:

Expression profiling by array

Platforms:

GPL2874 GPL892

24 Samples

Download data: TXT

(Submitter supplied) The hemibiotrophic fungus Magnaporthe oryzae produces specialized biotrophic invasive hyphae (IH) that alter membrane structure and defense responses in invaded rice cells. IH successively invade live neighbor cells, apparently through plasmodesmata. Understanding fungal and rice genes that contribute to biotrophic invasion has been a challenge because so few plant cells have encountered IH at the earliest infection stages. more...

Organism:

Oryza sativa; Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platform:

GPL2874

12 Samples

Download data: TXT

(Submitter supplied) The dark-phase in light/dark cycle plays an important role in successful disease development in M. oryzae-Oryza sativa. We used microarrays to detail the global programme of gene expression in response to light-to-dark transition. We identified different groups of genes (based on GO categories) that are up- or down-regulated in response to light-to-dark transition. Keywords: time course

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL889

15 Samples

Download data: TXT

(Submitter supplied) This series examines differential gene expression during appressorium formation induced by exogeous cyclic AMP in the rice blast fungus Magnaporthe grisea. RNA was extracted from spores germinated on hydrophilic (non-inductive) side of GelBond as well as from spores elaborating appressia on hydrophilic surfaces in the presence of cAMP after 9 hrs incubation. RNA from two biological replications of each treatment was pooled in equal amounts and labeled with both cy3 and cy5 dyes using the Agilent Technologies Low Input Linear Amplification Kit. more...

Organism:

Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL2874

4 Samples

Download data: TXT

(Submitter supplied) Rice blast, caused by the fungal pathogen Magnaporthe grisea, is a devastating disease causing tremendous yield loss in rice production. The public availability of the complete genome sequence of M. grisea provides ample opportunities to understand the molecular mechanism of its pathogenesis on rice plants at the transcriptome level. To identify all the expressed genes encoded in the fungal genome, we have analyzed the mycelium and appressorium transcriptomes using MPSS, RL-SAGE and oligoarray methods. more...

Organism:

Pyricularia grisea

Type:

Expression profiling by array; Expression profiling by SAGE

Platforms:

GPL4080 GPL4085

2 Samples

Download data: TXT

(Submitter supplied) This experiment was designed to analyze transcriptional changes that occur when the fungal plant pathogen M. grisea is shifted from a complete media to a nitrogen-starved media. Comparisons were made in the following way between fungal mycelial grown in minimal media supplemented with a nitrogen source (MM+N) and minimal media without a nitrogen source (NS) Keywords: parallel sample

Organism:

Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL889

6 Samples

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(Submitter supplied) This series examines differential gene expression during germination and appressorium formation by the rice blast fungus Magnaporthe grisea. Conidia were germinated on either the hydrophobic (inductive) or hydrophilic (non-inductive) side of GelBond. RNA was extracted from conidia and following incubation for 7 and 12 hours. RNA from two biological replications of each treatment was pooled in equal amounts and labeled with both cy3 and cy5 dyes using the Agilent Technologies Low Input Linear Amplification Kit. more...

Organism:

Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL889

10 Samples

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