GEO DataSets

(Submitter supplied) This experiment was designed to analyze transcriptional changes that occur when the fungal plant pathogen M. grisea is shifted from a complete media to a nitrogen-starved media. Comparisons were made in the following way between fungal mycelial grown in minimal media supplemented with a nitrogen source (MM+N) and minimal media without a nitrogen source (NS) Keywords: parallel sample

Organism:

Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL889

6 Samples

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(Submitter supplied) Transcriptional analysis using RNA-Seq to low oxygen

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16530

6 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by C2H2 transcription factors Vrf1 by RNA-seq in the rice blast fungus Magnaporthe oryzae Methods: Appressorial mRNAs of the wild type strain 70-15 and Δvrf1 conidia incubated in H2O on plastic membranes at 28˚C for 5 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae 70-15

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21479

6 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by C2H2 transcription factors Vrf1 and Vrf2 by RNA-seq and to evaluate the similarities and differences in gene regulation mechanisms of Vrf1 and Vrf2 in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, Δvrf1 and Δvrf2 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

9 Samples

Download data: TXT

(Submitter supplied) Rice blast, caused by the fungal pathogen Magnaporthe grisea, is a devastating disease causing tremendous yield loss in rice production. The public availability of the complete genome sequence of M. grisea provides ample opportunities to understand the molecular mechanism of its pathogenesis on rice plants at the transcriptome level. To identify all the expressed genes encoded in the fungal genome, we have analyzed the mycelium and appressorium transcriptomes using MPSS, RL-SAGE and oligoarray methods. more...

Organism:

Pyricularia grisea

Type:

Expression profiling by array; Expression profiling by SAGE

Platforms:

GPL4080 GPL4085

2 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by bHLH protein MoBhlh6 (Crf1) by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMobhlh6 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). RNA-sequencing libraries were constructed using NEBNext RNA sample preparation kit (NEB). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

6 Samples

Download data: TXT

(Submitter supplied) The rice blast fungus, Magnaporthe oryzae is a destructive pathogen of rice and other related crops, causing significant yield losses worldwide. Endogenous small RNAs (sRNAs), including small interfering RNAs (siRNAs) and microRNAs (miRNAs) are critical components of gene regulation in many eukaryotic organisms. Recently several new species of sRNAs have been identified in fungi. This fact along with the availability of genome sequence makes M. more...

Organism:

Oryza sativa; Pyricularia oryzae

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL9316 GPL16451

8 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by the regulatory factor X protein MoRfx1 by RNA-seq in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, ΔMorfx1 incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

6 Samples

Download data: TXT

(Submitter supplied) Analysis of RNA expression of Rhizoctonia solani AG1 IA. mRNA-seq of R. solani AG1 IA at 6 timepoints during the plant infection were sequenced. Cufflinks was used for calculating expected fragments per kilobase of transcript per million fragments sequenced (FPKM) values. Expression and regulation were identified. Analysis provides suggestion for discovering novel effectors and understanding pathogen factors.

Organism:

Rhizoctonia solani AG-1 IA

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15038

6 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to find out the genes regulated directly or indirectly by Zn2Cys6 transcription factors Cnf2 and Gpf1 by RNA-seq and to evaluate the similarities and differences in gene regulation mechanisms of Cnf2 and Gpf1 in the rice blast fungus Methods: Mycelial mRNAs of the wild type strain 70-15, mutants Δgpf1 (01F1-1) and Δcnf2 (03C7-2) incubated in H2O at 25˚C for 4 h, in triplicate independently for each strain, were extracted and isolated by RNeasy Plant mini kit (QIAGEN) and AMPure XP beads (Beckman). more...

Organism:

Pyricularia oryzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18618

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Expression profiling by array; Genome binding/occupancy profiling by array

Platforms:

GPL9203 GPL9182

19 Samples

Download data: GFF, PAIR, TXT

(Submitter supplied) The Ca2+/calcineurin signaling pathway is a central conduit regulating growth, development, and virulence of fungal pathogens infecting plants and human. We have analyzed global gene expression profiles during Ca2+ treatment in the rice blast fungus, Magnaporthe oryzae. An immunosuppressive drug, FK506, and the knock-out mutant of a transcription factor, MoCRZ1, were included to analyze calcineurin- and/or CRZ1-dependent gene expression, respectively. more...

Organism:

Pyricularia oryzae; Pyricularia grisea

Type:

Expression profiling by array

Platform:

GPL9203

16 Samples

Download data: TXT

(Submitter supplied) CRZ1 (Calcineurin Responsive Zinc finger 1) is a conserved transcription factor that relays Ca2+/calcineurin signals within the cell. To catalog genome wide locations that MoCRZ1 binds to, we have conducted chromatin immunoprecipitation coupled with microarray (ChIP-chip) in the rice blast fungus, Magnaporthe oryzae. Fungal mycelia were treated with 200mM CaCl2 to activate translocation of MoCRZ1 to the nucleus, thereby binding to the promoter regions of downstream genes, and CaCl2 together with 10ug/ml FK506 to block these phenomena. more...

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Genome binding/occupancy profiling by array

Platform:

GPL9182

3 Samples

Download data: GFF, PAIR

(Submitter supplied) Rice blast is the most threatening disease to cultivated rice worldwide. Magnaporthe oryzae, its causal agent is likely to encounter environmental challenges during invasive growth in the host plants that require shifts in gene expression to establish a compatible interaction. Here, we tested the hypothesis that M. oryzae have similar gene expression patterns in planta, versus during in vitro stresses. more...

Organism:

Pyricularia grisea; Pyricularia oryzae

Type:

Expression profiling by array

Platform:

GPL9203

16 Samples

Download data: TXT

(Submitter supplied) Gene expression analysis of A. parasiticus grown under conditions conducive and nonconductive for aflatoxin production was evaluated using glass slide microarrays containing 765 ESTs. Aflatoxins (AF) produced by Aspergillus flavus and A. parasiticus are responsible for approximately $270M in losses annually in the corn, peanut, cotton, and tree nut industries of the United States. In an attempt to develop effective control procedures, research has focused on elucidating the pathway of aflatoxin biosynthesis with the goal of identifying potential targets for intervention. more...

Organism:

Aspergillus flavus; Aspergillus parasiticus

Type:

Expression profiling by array

Platforms:

GPL1394 GPL1393

16 Samples

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(Submitter supplied) The Transcriptome of different tissues and developmental stages of Laccaria bicolor S238N was analyzed. The array probes were designed from gene models taken from the Joint Genome institute Laccaria bicolor genome sequence version1. One aim of this study was to verify the expression of the automatically annotated gene models in various tissues and to use this transcriptional information to confirm, to correct or to reject gene models. more...

Organism:

Populus trichocarpa; Laccaria bicolor; Pseudotsuga menziesii; Populus tremula x Populus alba; Laccaria bicolor S238N-H82

Type:

Expression profiling by array

Platform:

GPL6192

11 Samples

Download data: TXT