GEO DataSets

(Submitter supplied) Bacillus anthracis, the causative agent of anthrax, secretes three toxin proteins: protective antigen (PA), lethal factor (LF), and edema factor (EF). PA is a transporter of LF and EF into host cells by receptor-mediated endocytosis. LF is a metalloprotease that cleaves mitogen-activated protein kinase (MAPK) kinases (MKK), while EF is an adenylate cyclase, which converts ATP to cAMP. We used microarrays to decipher the specific gene regulation in edema toxin (ET), the complex of EF and PA, treated mouse bone marrow derived macrophages. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

3 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3’,5’-monophosphate, resulting in interstitial edema seen in anthrax patients. We used GeneChip analysis to examine global transcriptional profiles of EdTx-treated RAW 264.7 murine macrophage-like cells at 3 and 6 hr. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2410

Platform:

GPL1261

21 Samples

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Analysis of RAW 264.7 macrophage cells treated with edema toxin (EdTx) for 3 or 6 hours. EdTx is an adenylyl cyclase enzyme produced by Bacillus anthracis. It converts ATP to cAMP, resulting in interstitial edema in anthrax patients. Results provide insight into the effect of EdTx in the host cell.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 agent, 3 time sets

Platform:

GPL1261

Series:

GSE4712

21 Samples

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(Submitter supplied) Edema factor of anthrax binds to the protective antigen (PA83) which cleaves to PA63 and PA20. The role of PA20 was examined using highthroughput gene expression analysis of human peripheral blood mononuclear cells (PBMC) exposed to PA20. Keywords: Toxicity determination

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3033

14 Samples

Download data: GPR