GEO DataSets

(Submitter supplied) Transcriptional profiling of H37Rv (WT), Mut1 and Comp1 bacteria under aerobic (Aer/0 day, i.e 0 D) and hypoxic conditions (Hyp/5 days standing culture, i.e 5 D). Mut1: H37Rv carrying devR gene disruption by in frame insertion of kanamycin resistance cassette and expressing DevRN-Kan fusion protein. Comp1: Mut1 complemented with low copy number plasmid carrying devR gene expressed from its native constitutive upstream promoter. more...

Organism:

Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL13729

17 Samples

Download data: TXT

(Submitter supplied) Transcriptional profile comparison among PDIM positive and negative isolates. Standard in vitro exponential phase cultures of M. tuberculosis H37Rv ATCC27294 were plated on 7H11 plates. Individual colonies were sub-cultured in 7H9 broth and metabolically labeled with [14C] propionic acid. The apolar lipid fraction was then extracted from those cultures and assayed for PDIM (phthiocerol dimycocerosate) content via TLC. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL8893

6 Samples

Download data: TXT

(Submitter supplied) Genomic comparisons among PDIM positive and negative isolates. Standard in vitro exponential phase cultures of M. tuberculosis H37Rv ATCC27294 were plated on 7H11 plates. Individual colonies were sub-cultured in 7H9 broth and metabolically labeled with [14C] propionic acid. The apolar lipid fraction was then extracted from those cultures and assayed for PDIM (phthiocerol dimycocerosate) content via TLC. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL8893

4 Samples

Download data: TXT

(Submitter supplied) The present study reports the gene expression data of Mycobacterium tuberculosis H37Rv and H37RvΔdosSΔdosT (DKO) grown on 0.2 % acetate/glucose under aerobic/hypoxic conditions. Acetate was reported to be present in granulomas of Mycobacterium tuberculosis infected guinea pigs which are also hypoxic. By exposing Mycobacterium tuberculosis H37Rv and H37RvΔdosSΔdosT to different combinations of granulomatous stresses (acetate/glucose and aerobic/hypoxic conditions) alongwith other experimental data, we were able to delineate a new signaling pathway that activates DevR (DosR) regulon through Acetyl phosphate. more...

Organism:

Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL21002

12 Samples

Download data: TXT

(Submitter supplied) Transcriptional profile comparison among Beijing and non-Beijing M. tuberculosis isolates.

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL8893

6 Samples

Download data: TXT

(Submitter supplied) In Mycobacterium tuberculosis, the sensor kinases DosT and DosS activate the transcriptional regulator DosR, resulting in the induction of the DosR regulon, important for anaerobic survival and perhaps latent infection. The individual and collective roles of these sensors has been postulated biochemically, but their roles have remained unclear in vivo. This work demonstrates distinct and additive roles for each sensor during anaerobic dormancy. more...

Organism:

Mycobacterium tuberculosis H37Rv; Mycobacterium tuberculosis CDC1551

Type:

Expression profiling by array

Platform:

GPL8768

36 Samples

Download data: GPR

(Submitter supplied) Transcription analysis of ΔnarL mutant (LIX75) versus wild type H37Rv during aerobic growth in absence or presence of 5 mM Nitrate, and during hypoxic growth. This will elucidate the regulatory role of NarL response regulator duirng aerobic growth in absence of presence of exogenous nitrate supplemen and during hypoxic growth conditions and help identify genes that require NarL for expression under the three experimental conditions.

Organism:

Mycobacterium tuberculosis; Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL15500

6 Samples

Download data: TXT

(Submitter supplied) In-vivo Gene Signatures of Mycobacterium tuberculosis In C3HeB/FeJ Mice

Organism:

Mycobacterium tuberculosis CDC1551; Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL18320

12 Samples

Download data: GPR

(Submitter supplied) Here we are including replicate mouse microarray data from Kramnik mice infected over the course of different time points with M. tuberculosis H37Rv and its isogenic mutants in dosR, dosS and dosT genes.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

24 Samples

Download data: GPR

(Submitter supplied) A M. tuberculosis transposon library was used to infect WT and iNOS-/- mice. Surviving mutants were recovered from spleens, genomic DNA was extracted, and labeled probes were synthesized from transposon ends. Probes from each WT mouse were hybridized with probes from a similar iNOS-/- mouse.

Organism:

Mycobacterium tuberculosis

Type:

Other

Platform:

GPL9051

26 Samples

Download data: GPR

(Submitter supplied) The purpose of this study was to examine how ethoxzolamide modulates gene M. tuberculosis gene expression at acidic pH. We observed that ethoxzolamide downregulates genes of the PhoPR regulon.

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17879

8 Samples

Download data: TXT

(Submitter supplied) Background: A significant body of evidence accumulated over the last century suggests a link between hypoxic microenvironments within the infected host and the latent phase of tuberculosis. Studies to test this correlation have identified the M. tuberculosis initial hypoxic response, controlled by the two-component response regulator DosR. The initial hypoxic response is completely blocked in a dosR deletion mutant. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platforms:

GPL5774 GPL4293 GPL4291

52 Samples

Download data: GPR

(Submitter supplied) To survive a dynamic host environment, Mycobacterium tuberculosis must endure a series of challenges from reactive oxygen and nitrogen stress, to drastic shifts in oxygen availability. The mycobacterial Lsr2 protein has been implicated in reactive oxygen defense via direct protection of DNA. To examine the role of Lsr2 in pathogenesis and physiology of M. tuberculosis, we generated a strain deleted for lsr2. more...

Organism:

Mycobacterium tuberculosis; Mycobacterium tuberculosis CDC1551; Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL15398

16 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of M.tuberculosis to 10 mM vitamin C at 8 h. This was compared to gene expression profile of untreated M. tuberculosis culture.

Organism:

Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL18055

7 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mycobacterium tuberculosis CDC1551; Macaca mulatta; Mycobacterium tuberculosis; Homo sapiens; Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platforms:

GPL18320 GPL10183

14 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of bacterial RNA samples from infected Rhesus Bone Marrow Derived Macrophages (RhBMDMs) compared to RNA isolated from respective in vitro grwon cultures viz M. tuberculosis H37Rv or Isogeneic mutant MtbdosS

Organism:

Mycobacterium tuberculosis CDC1551; Mycobacterium tuberculosis; Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL18320

6 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of RNA samples from un-infected Rhesus Bone Marrow Derived Macrophages (RhBMDMs) exposed to IMDM complete media used as control were compared to RNA from RhBMDMs infected for 24 hr with 'dos' mutants of Mtb viz. MtbΔdosR, MtbΔdosS, or MtbΔdosT

Organism:

Homo sapiens; Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL10183

8 Samples

Download data: TXT

(Submitter supplied) We have shown that elevated levels of wild-type M. tuberculosis PknK (PknK Mtb), but not phosphorylation-defective PknKMtb, in Mycobacterium smegmatis cause significant retardation of growth rate and altered colony morphology (Malhotra et al., 2012). LIX79 (WT PknK) and LIX80 (PknK K55M mutant) M. smegmatis strains will be grown in LB and induced with 0.2% acetamide for the induction of wild type or phosphorylation defective pknK gene. more...

Organism:

Mycolicibacterium smegmatis

Type:

Expression profiling by array

Platform:

GPL30399

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mycobacterium tuberculosis H37Rv; Mycolicibacterium smegmatis MC2 155

Type:

Expression profiling by array

Platforms:

GPL14580 GPL14591

2 Samples

Download data: GPR

(Submitter supplied) Transcriptional profile of Mycobacterium tuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress.

Organism:

Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL14591

1 Sample

Download data: GPR