GEO DataSets

(Submitter supplied) Acute myeloid leukemia (AML) is a heterogeneous disease in respect of molecular aberrations and prognosis. We used gene expression profiling of 562 patients treated in the German AMLCG 1999 trial to develop a gene signature that predicts survival in AML.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL570 GPL97

984 Samples

Download data: CEL, TXT

(Submitter supplied) Primary therapy resistance is a major problem in acute myeloid leukemia treatment. We set out to develop a powerful and robust predictor for therapy resistance for intensively treated adult patients. We used two large gene expression data sets (n=856) to develop a predictor of therapy resistance, which was validated in an independent cohort analyzed by RNA sequencing (n=250). In addition to gene expression markers, standard clinical and laboratory variables as well as the mutation status of 68 genes were considered during construction of the model. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18460

250 Samples

Download data: TXT, XLSX

(Submitter supplied) Mononuclear cells from AML patients (n=46) were sorted into CD34+ and CD34- subfractions and genome-wide expression analysis was performed using Illumina BeadChip Arrays (HT12 v3). Of 2 AML samples only the CD34+ fraction could be analyzed. AML CD34+ and CD34- gene expression was compared to a large group of normal CD34+ bone marrow cells (n=31).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

121 Samples

Download data: TXT

(Submitter supplied) The aim of the study was to get insights into transcriptional alterations in bone marrow mesenchymal stromal cells derived from acute myeloid leukemia patients We compared the global gene expression profile from AML BM-MSC (n=19) to healthy donor (HD) controls (HD BM-MSC n=4)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

23 Samples

Download data: CEL

(Submitter supplied) Genome wide methylation profiling of BM-MSC derived from AML patients in comparison to healthy donor controls using Illumina Infinium HumanMethylation450 Beadchip

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL13534

44 Samples

Download data: IDAT, TXT

(Submitter supplied) In leukemias and other malignancies of the bone marrow, little is known about the fate of fibroblasts and resident macrophages after normal hematopoietic cells are replaced by neoplastic cells. In the present investigation we used two-stage long-term bone marrow cultures to detect functional stromal cell abnormalities in acute myeloid leukemia, myelodysplastic syndromes and multiple myeloma. While fibroblasts from multiple myeloma and macrophages from multiple myeloma and myelodysplastic syndromes were functionally indistinguishable from the respective cell types from normal bone marrow, fibroblasts from patients with acute myeloid leukemia or myelodysplastic syndromes possessed a significantly lower ability to support hematopoiesis originating from co-cultured normal CD34-positive cells than fibroblasts from healthy marrow. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

16 Samples

Download data: CEL, CHP

(Submitter supplied) Long noncoding RNAs (lncRNAs) are transcripts longer than 200 nucleotides located within the intergenic stretches or overlapping antisense transcripts of protein coding genes. LncRNAs are involved in numerous biological roles including imprinting, epigenetic regulation, apoptosis and cell-cycle. To determine whether lncRNAs are associated with clinical features and recurrent mutations in older patients (aged ≥60 years) with cytogenetically normal (CN) acute myeloid leukemia (AML), we evaluated lncRNA expression in 148 untreated older CN-AML cases using a custom microarray platform.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

71 Samples

Download data: XLS

(Submitter supplied) Long noncoding RNAs (lncRNAs) are transcripts longer than 200 nucleotides located within the intergenic stretches or overlapping antisense transcripts of protein coding genes. LncRNAs are involved in numerous biological roles including imprinting, epigenetic regulation, apoptosis and cell-cycle. To determine whether lncRNAs are associated with clinical features and recurrent mutations in older patients (aged ≥60 years) with cytogenetically normal (CN) acute myeloid leukemia (AML), we evaluated lncRNA expression in 148 untreated older CN-AML cases using a custom microarray platform.

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL16956

148 Samples

Download data: TXT

(Submitter supplied) Global gene expression comparison between mesenchymal stem cells (MSCs) purified from the BM of AML patients versus healthy donors.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

12 Samples

Download data: CEL

(Submitter supplied) Acute myeloid leukemia (AML) is a genetically heterogeneous disease that is characterized by abnormal clonal proliferation of myeloid progenitor cells found predominately within the bone marrow (BM) and blood. Recent studies suggest that genetic and phenotypic alterations in the BM microenvironment support leukemogenesis and allow leukemic cells to survive and evade chemotherapy-induced death. However, despite substantial evidence indicating the role of tumor-host interactions in AML pathogenesis, little is known about the complex microenvironment of the BM. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21290

19 Samples

Download data: CSV

(Submitter supplied) Patients with cytogenetically normal acute myeloid leukemia (CN-AML) show heterogeneous treatment outcomes. We used gene expression profiling to develop a gene signature that predicts overall survival (OS) in CN-AML. Based on data from 163 patients treated in the German AMLCG 1999 trial and analyzed on oligonucleotide microarrays, we used supervised principal component analysis to identify 86 probe sets (representing 66 different genes) which correlated with OS, and defined a prognostic score based on this signature. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS3308 GDS3312 GDS3329

Platforms:

GPL570 GPL96 GPL97

405 Samples

Download data: CEL

Analysis of mononuclear cells from bone marrow or peripheral blood from a test set of adult patients with cytogenetically normal acute myeloid leukemia (CN-AML). CN-AML patients show heterogeneous treatment outcomes. Results provide insight into the prognostic value of a gene signature for CN-AML.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 tissue sets

Platform:

GPL570

Series:

GSE12417

79 Samples

Download data: CEL

Analysis of mononuclear cells from bone marrow or peripheral blood from a training set of 163 adult patients with cytogenetically normal acute myeloid leukemia (CN-AML). Patients with CN-AML show heterogeneous treatment outcomes. Results provide insight into a prognostic gene signature for CN-AML.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 tissue sets

Platform:

GPL96

Series:

GSE12417

163 Samples

Download data: CEL

Analysis of mononuclear cells from bone marrow or peripheral blood from a training set of 163 adult patients with cytogenetically normal acute myeloid leukemia (CN-AML). Patients with CN-AML show heterogeneous treatment outcomes. Results provide insight into a prognostic gene signature for CN-AML.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 tissue sets

Platform:

GPL97

Series:

GSE12417

163 Samples

Download data: CEL

(Submitter supplied) Purpose: CEBPA mutations are found as either biallelic (biCEBPA) or monoallelic (moCEBPA). We set out to explore whether the kind of CEBPA mutation is of prognostic relevance in cytogenetically normal AML (CN-AML). Patients and Methods: 467 homogeneously treated CN-AML patients were subdivided into moCEBPA, biCEBPA and wildtype (wt) CEBPA patients. The subgroups were analyzed for clinical parameters and for additional mutations in the NPM1, FLT3 and MLL genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8289

61 Samples

Download data: CEL

(Submitter supplied) Acute myeloid leukemia (AML) cells can shape their niche to their own advantage, perturbing bone marrow stromal and immune landscape. Indeed, AML cells provide the signals, among which inflammatory mediators are crucial, since they are able to subvert mesenchymal stromal cell (MSC) funtions. In particular, IFN-γhigh AML cells hold an inflammatory/immune modulating signature distinct from IFN-γlow cases. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23159

16 Samples

Download data: CEL

(Submitter supplied) Pirin (PIR) is a putative transcriptional regulator whose expression is silenced in cells bearing the AML1/ETO and PML/RAR leukemogenic fusion proteins and is significantly repressed in a large proportion of acute myeloid leukemias. PIR expression increases during in vitro myeloid differentiation of primary hematopoietic precursor cells, and ablation of PIR in the U937 myelomonocytic cell line or in murine primary hematopoietic precursor cells results in impairment of terminal myeloid differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5254

Platform:

GPL570

4 Samples

Download data: CEL

Analysis of U937 myelomonocytic cells depleted for Pirin. Pirin is silenced in cells with the acute myeloid leukemia-1 eight-twenty-one and promyelocytic leukemia/retinoic acid receptor leukemogenic fusion proteins. Results provide insight into the role of Pirin in myeloid differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL570

Series:

GSE16798

4 Samples

Download data: CEL

(Submitter supplied) Context: In many cancers, specific subpopulations of cells appear to be uniquely capable of initiating and maintaining tumors. The strongest support for this cancer stem cell model comes from transplantation assays in immune-deficient mice indicating that human acute myeloid leukemia (AML) is organized as a cellular hierarchy driven by self-renewing leukemia stem cells (LSC). This model has significant implications for the development of novel therapies, but its clinical significance remains unclear. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL10881

54 Samples

Download data: CEL, TXT

(Submitter supplied) Murine models of myeloid neoplasia show how leukemia infiltration alters the mesenchymal stem and progenitor cells to reinforce malignancy at the expense of healthy hematopoiesis. However, little is known about the bone marrow architecture in humans. We used global gene expression analyses to analyze mesenchymal stem and progenitor cells from AML patients and compared with mesenchymal stem and progenitor cells from the non-leukemic donor.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23159

11 Samples

Download data: CEL, CHP