daf-9 3-ketosteroid oxygenase;Cytochrome P450 [Caenorhabditis elegans] - Gene

Summary

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Gene symbol: daf-9
Gene description: 3-ketosteroid oxygenase;Cytochrome P450
Locus tag: CELE_T13C5.1
See related: AllianceGenome:WB:WBGene00000905
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable heme binding activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; and steroid hydroxylase activity. Involved in cell-cell signaling; dauer larval development; and regulation of cell migration. Located in neuronal cell body and perinuclear region of cytoplasm. Is expressed in several structures, including anterior ganglion; excretory cell; head neurons; spermatheca; and vulval muscle. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See daf-9 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 12

Sequence:: Chromosome: X; NC_003284.9 (6196927..6200823)

Chromosome X - NC_003284.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003284.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Expression of DAF-9 is increased in C. elegans subjected to dietary restriction.
Title: Steroid hormone signalling links reproduction to lifespan in dietary-restricted Caenorhabditis elegans.
Results altogether suggest that circulatory mutual regulation among daf-9, daf-12, and daf-16 at the expression level mediates cholesterol signal to control larval development upon cholesterol starvation.
Title: A circulatory transcriptional regulation among daf-9, daf-12, and daf-16 mediates larval development upon cholesterol starvation in Caenorhabditis elegans.
We propose that the dauer rescuing activity is a hormone synthesized by DAF-9 that acts through DAF-12.
Title: Lipophilic regulator of a developmental switch in Caenorhabditis elegans.
results support the hypothesis that ncr-1 and ncr-2 are involved in intracellular cholesterol processing in C. elegans, and that a sterol-signaling defect is responsible for the Daf-c phenotype of the ncr-2; ncr-1 mutant
Title: NCR-1 and NCR-2, the C. elegans homologs of the human Niemann-Pick type C1 disease protein, function upstream of DAF-9 in the dauer formation pathways.
We identified two distinct 3-keto-cholestenoic acid metabolites of DAF-9, a cytochrome P450 involved in hormone production, that function as ligands for DAF-12.
Title: Identification of ligands for DAF-12 that govern dauer formation and reproduction in C. elegans.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T13C5.1 (e-PCR)
- UniSTS:310833

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables heme binding	IBA Inferred from Biological aspect of Ancestor more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen	IBA Inferred from Biological aspect of Ancestor more info
enables steroid hydroxylase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cell-cell signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cholesterol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in dauer larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in organic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in steroid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in xenobiotic metabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 3-ketosteroid oxygenase;Cytochrome P450

NP_001024903.1

Confirmed by transcript evidence

NP_001362132.1

Confirmed by transcript evidence

NP_741807.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003284.9 Reference assembly

Range

6196927..6200823

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_171699.6 → NP_741807.2 3-ketosteroid oxygenase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_741807.2

Status: REVIEWED

UniProtKB/Swiss-Prot

G5ECA9, H2KYS3

Conserved Domains (1) summary

pfam00067
Location:108 → 559

p450; Cytochrome P450
NM_001029732.3 → NP_001024903.1 3-ketosteroid oxygenase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001024903.1

Status: REVIEWED

UniProtKB/Swiss-Prot

H2KYS3

Conserved Domains (1) summary

pfam00067
Location:93 → 544

p450; Cytochrome P450
NM_001375044.2 → NP_001362132.1 Cytochrome P450 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A5E4M3J0

Conserved Domains (1) summary

pfam00067
Location:72 → 523

p450; Cytochrome P450