top2a DNA topoisomerase II alpha [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: top2aprovided by ZNC
Official Full Name: DNA topoisomerase II alphaprovided by ZNC
Primary source: ZFIN:ZDB-GENE-030131-2453
See related: Ensembl:ENSDARG00000024488 AllianceGenome:ZFIN:ZDB-GENE-030131-2453
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: fc08c07; wu:fc08c07
Summary: Predicted to enable DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity. Acts upstream of or within animal organ regeneration and chromosome segregation. Predicted to be located in nucleoplasm. Predicted to be part of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex. Predicted to be active in nucleus. Is expressed in digestive system; head; nervous system; neural tube; and pectoral fin. Orthologous to human TOP2A (DNA topoisomerase II alpha). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 12

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
106	current	GRCz11 (GCF_000002035.6)	12	NC_007123.7 (10706772..10740236)
105	previous assembly	GRCz10 (GCF_000002035.5)	12	NC_007123.6 (10668469..10701933)

Chromosome 12 - NC_007123.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The absence of zygotic top2a is not fully compensated for by maternal, zygotic or ectopic top2b mRNA suggesting distinct functions of Top2a and Top2b in embryonic development.
Title: Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants.
Results confirm a conserved role for topoisomerase II alpha (TOP2A) in vertebrates as well as a dose-sensitive requirement for top2a in adults.
Title: Topoisomerase II alpha is required for embryonic development and liver regeneration in zebrafish.

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General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	ISS Inferred from Sequence or Structural Similarity more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	ISS Inferred from Sequence or Structural Similarity more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA topological change	IEA Inferred from Electronic Annotation more info
involved_in DNA topological change	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA topological change	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within animal organ regeneration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chromosome condensation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chromosome segregation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within mitotic S phase	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in resolution of meiotic recombination intermediates	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within rhythmic process	IEA Inferred from Electronic Annotation more info
involved_in sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: DNA topoisomerase 2-alpha

Names: topoisomerase (DNA) II alpha; topoisomerase 2

NP_001296944.1

EC 5.6.2.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001310015.1 → NP_001296944.1 DNA topoisomerase 2-alpha

Status: VALIDATED

Source sequence(s)

BX470262, EB928159, EH541591, EH551427, FO704786

UniProtKB/TrEMBL

A0A0R4IUF0, A0A8M1P7U6

Related

ENSDARP00000139353.2, ENSDART00000158227.2

Conserved Domains (7) summary

cd03365
Location:454 → 574

TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...

cd00187
Location:710 → 1166

TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...

cd03481
Location:264 → 416

TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...

PTZ00108
Location:25 → 1440

PTZ00108; DNA topoisomerase 2-like protein; Provisional

pfam02518
Location:79 → 173

HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

pfam08070
Location:1468 → 1560

DTHCT; DTHCT (NUC029) region

pfam16898
Location:572 → 710

TOPRIM_C; C-terminal associated domain of TOPRIM

RefSeqs of Annotated Genomes: Danio rerio Annotation Release 106 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007123.7 Reference GRCz11 Primary Assembly

Range

10706772..10740236

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_001003834.1: Suppressed sequence

Description

NM_001003834.1: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.