ATG21 Atg21p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ATG21
Gene description: Atg21p
Primary source: FungiDB:YPL100W
Locus tag: YPL100W
See related: SGD:S000006021; AllianceGenome:SGD:S000006021; VEuPathDB:YPL100W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HSV1; MAI1
Summary: Enables phosphatidylinositol-3,5-bisphosphate binding activity; phosphatidylinositol-3-phosphate binding activity; and phosphatidylinositol-4-phosphate binding activity. Involved in several processes, including cytoplasm to vacuole transport by the Cvt pathway; piecemeal microautophagy of the nucleus; and protein localization to phagophore assembly site. Located in several cellular components, including endosome; fungal-type vacuole membrane; and phagophore assembly site. Orthologous to human WIPI1 (WD repeat domain, phosphoinositide interacting 1) and WIPI2 (WD repeat domain, phosphoinositide interacting 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ATG21 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (361872..363362)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ATG21 is necessary for cell death induced by oxidants. Atg21 interacts with Rho5 preferentially in its GTP-bound state, suggesting that Atg21 is a downstream target of Rho5 in oxidant-induced cell death.
Title: Genome-Wide Studies of Rho5-Interacting Proteins That Are Involved in Oxidant-Induced Cell Death in Budding Yeast.
Atg21 thus defines PI3P-dependently the lipidation site by linking and organising the E3 ligase complex and Atg8 at the PAS.
Title: PI3P binding by Atg21 organises Atg8 lipidation.
lipid-binding motifs of Atg18 and Atg21 have roles in the cytoplasm to vacuole targeting pathway and autophagy
Title: Roles of the lipid-binding motifs of Atg18 and Atg21 in the cytoplasm to vacuole targeting pathway and autophagy.
Deletion of ATG18, ATG21 and YGR223c, alone or simultaneously has no obvious function on the MVB-pathway and carboxypeptidase Y sorting.
Title: Dissecting the localization and function of Atg18, Atg21 and Ygr223c.
Mutated Atg21-(FTTGT) is inactive during the Cvt pathway but showed only partly reduced binding to PtdIns-phosphates, suggesting further lipid binding domains in Atg21
Title: The relevance of the phosphatidylinositolphosphat-binding motif FRRGT of Atg18 and Atg21 for the Cvt pathway and autophagy.
Atg21 is involved in the recruitment of membrane to the pre-autophagosomal structure.
Title: Atg21 is a phosphoinositide binding protein required for efficient lipidation and localization of Atg8 during uptake of aminopeptidase I by selective autophagy.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol-3,5-bisphosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-3,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-3-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in autophagy of mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagy of mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagy of peroxisome	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in macroautophagy	IEA Inferred from Electronic Annotation more info
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in piecemeal microautophagy of the nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein lipidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein lipidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to phagophore assembly site	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to phagophore assembly site	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in phagophore assembly site	IDA Inferred from Direct Assay more info	PubMed
located_in phagophore assembly site	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in phagophore assembly site membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Atg21p

NP_015225.1

Phosphoinositide binding protein; required for vesicle formation in cytoplasm-to-vacuole targeting (CVT) pathway, autophagy, micronucleophagy and mitophagy; binds to several phosphoinositides including PtdIns(3,5)P2, PI3P and PI4P; involved in PI3P-dependent recruitment and organization of both Atg12-Atg5-Atg16 complex and Atg8p at pre-autophagosomal structure; necessary for oxidant-induced cell death; targeted to vacuole via AP-3 pathway; WD-40 repeat containing PROPPIN family member

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

361872..363362

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183914.1 → NP_015225.1 TPA: Atg21p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015225.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3R6, Q02887

UniProtKB/TrEMBL

A0A8H4BU44, B3LKX2, C8ZIT0, N1NX54

Conserved Domains (2) summary

smart00320
Location:295 → 320

WD40; WD40 repeats

sd00039
Location:300 → 343

7WD40; WD40 repeat [structural motif]