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| Status |
Public on Sep 28, 2018 |
| Title |
NONO detects the nuclear HIV capsid to promote cGAS-mediated innate immune activation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Detection of viruses by innate immune sensors induces protective antiviral immunity. The viral DNA sensor cGAS is necessary for detection of HIV by human dendritic cells and macrophages. However, synthesis of HIV DNA during infection is not sufficient for immune activation. The capsid protein, which associates with viral DNA, has a pivotal role in enabling cGAS-mediated immune activation. We now find that NONO is an essential sensor of the HIV capsid in the nucleus. NONO protein directly binds capsid with higher affinity for weakly pathogenic HIV-2 than highly pathogenic HIV-1. Upon infection, NONO is essential for cGAS activation by HIV and cGAS association with HIV DNA in the nucleus. NONO recognizes a conserved region in HIV capsid with limited tolerance for escape mutations. Detection of nuclear viral capsid by NONO to promote DNA sensing by cGAS reveals an innate strategy to achieve distinction of viruses from self in the nucleus.
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| Overall design |
Monocyte-derived dendritic cells from healthy donors or NONO-deficient patients were either untouched, infected by a HIV-1 reporter virus with Vpx, infected by a HIV-2 GFP-reporter virus, or treated by cGAMP with lipofectamin. After 24 hours, RNA was extracted for gene expression analysis.
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| Contributor(s) |
Lahaye X, Nadalin F, Manel N |
| Citation(s) |
30270045 |
| Submission date |
Jan 24, 2018 |
| Last update date |
Dec 28, 2018 |
| Contact name |
Nicolas Manel |
| E-mail(s) |
nicolas.manel@curie.fr
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| Organization name |
Institut Curie
|
| Department |
INSERM U932
|
| Street address |
12 rue Lhomond
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL17692 |
[HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA431379 |
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