|
| Status |
Public on Sep 28, 2018 |
| Title |
MDDC-NI-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Dendritic cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Dendritic cells
genotype: NONO-KO
treatment: control
|
| Treatment protocol |
100 000 cells were left untouched, infected with HIV-1 with Vpx, infected with HIV-2, or treated with cGAMP and lipofectamin, for 24 hours.
|
| Growth protocol |
CD14+ cells isolated from human blood followed by differentiation with IL-4 and GM-CSF for 4 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Machery-Nagel NucleoSpin RNA extraction kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Nugen WT Ovation protocol using 500pg as starting amount.
|
| |
|
| Hybridization protocol |
Following fragmentation, 2,5 ug of cDNA were hybridized for 16 hr at 48°C onto Affymetrix Human Gene ST 1.1 array plate. Array plate was hybridized, washed and stained using an an Affymetrix Genetitan 4C device.
|
| Scan protocol |
Array plate was scannned using the Affymetrix Genetitan 4C device.
|
| Description |
Gene expression data from non-infected controls in NONO KO cells
|
| Data processing |
Raw data were treated, normalized and summarized using oligo R package.
|
| |
|
| Submission date |
Jan 24, 2018 |
| Last update date |
Sep 28, 2018 |
| Contact name |
Nicolas Manel |
| E-mail(s) |
nicolas.manel@curie.fr
|
| Organization name |
Institut Curie
|
| Department |
INSERM U932
|
| Street address |
12 rue Lhomond
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE109554 |
NONO detects the nuclear HIV capsid to promote cGAS-mediated innate immune activation |
|
