|
| Status |
Public on Mar 20, 2019 |
| Title |
Silencing of lnc-klhdc7b in the HCC1187 cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
By using shRNAs the lnc-klhdc7b was silenced in the cell line HCC1187, the shRNA-1 carried a ~75% silencing compared to the negative control (NC).
We evaluated the role of LncKLHDC7B on oncogenic phenotypes in the IM breast cancer HCC1187 cell line model. The knockdown of LncKLHDC7B was achieved with ~75% of silencing efficiency. A complete genomic analysis of the transcriptomic state after silencing of LncKLHDC7B revealed an impact on the global expression of transcripts
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| |
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| Overall design |
We analyzed expression profile from HCC1187 cell line silenced by shRNA-1 versus lnc-KLHDC7B using the Affymetrix Human Transcriptome Array 2.0 platform. Array data was processed by Affymetrix Exon Array Computational Tool.
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| |
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| Contributor(s) |
Hidalgo-Miranda A, Beltrán-Anaya FO |
| Citation(s) |
30648789 |
| Submission date |
May 15, 2018 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Alfredo Hidalgo-Miranda |
| E-mail(s) |
ahidalgo@inmegen.gob.mx
|
| Organization name |
INMEGEN
|
| Department |
Cancer Genomic
|
| Lab |
Cancer Genomic
|
| Street address |
Periferico Sur #4809
|
| City |
Ciudad de México |
| State/province |
CDMX |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
| |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
|
| Samples (6)
|
| GSM3142328 |
HCC1187 with shRNA-NC (negative control) rep1 |
| GSM3142329 |
HCC1187 with shRNA-NC (negative control) rep2 |
| GSM3142330 |
HCC1187 with shRNA-NC (negative control) rep3 |
|
| Relations |
| BioProject |
PRJNA471504 |
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