|
| Status |
Public on Mar 20, 2019 |
| Title |
HCC1187 with shRNA-NC (negative control) rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Negative Control
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: RNA from HCC1187 transfected with shRNA negative control
|
| Treatment protocol |
HCC1187 cell line was transfected with 3 μg of plasmid (shRNA-1) to silence lnc-KLHDC7B. As negative control (NC) a shRNA directed to GFP was transfected.
|
| Growth protocol |
The hcc1187 cell line was grown in RPMI + 10% fetal bovine serum prior to treatment with shRNAs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method was used to extract the total RNA
|
| Label |
biotin
|
| Label protocol |
SensationPlus™ FFPE Amplification and WT Labeling Kit were used to generate the biotinylated cRNA starting from 100ng of total RNA according to the manufacturer (Manual Target preparation for GeneChip Whole transcript (WT) expression arrays, Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Transcriptome Array 2.0 Arrays (Affymetrix)
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix). GeneChips were scanned using The Genechip Scanner 3000 7G (Affymetrix , Santa Clara, USA).
|
| Description |
SST-RMA expression value derived from Expression Console software
|
| Data processing |
Array signal intensitive were analyzed with the Affymetrix Expression Console using Signal Space Transformation-Robust Multichip Analysis (SST-RMA) for the background correction.
|
| |
|
| Submission date |
May 15, 2018 |
| Last update date |
Mar 20, 2019 |
| Contact name |
Alfredo Hidalgo-Miranda |
| E-mail(s) |
ahidalgo@inmegen.gob.mx
|
| Organization name |
INMEGEN
|
| Department |
Cancer Genomic
|
| Lab |
Cancer Genomic
|
| Street address |
Periferico Sur #4809
|
| City |
Ciudad de México |
| State/province |
CDMX |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE114468 |
Silencing of lnc-klhdc7b in the HCC1187 cell line |
|
