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| Status |
Public on Aug 12, 2019 |
| Title |
Bone marrow TTP deficeincy significantly increased inflammation while reduced plasma lipid and hepatic steatosis in LDLR knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to examine the effects of bone marrow TTP deficiency on inflammation and lipid metabolism and determine the driving force during atherosclerosis, 8 week-old female LDLR−/− mice on C57BL/6J background were lethally irradiated and reconstituted with a wild type (TTP+/+) or TTP knockout (TTP−/−) bone marrow cells and fed a Western diet for 12 weeks. Then mice were sacrificed and tissues were collected for analysis. Liver tissues were collected and gene expression was analysed using a whole genome microarray. Bone marrow TTP−/− recipients displayed a significantly higher systemic and multi-organ inflammation compared to the BM-TTP+/+ recipients. BM-TTP−/− mice displayed a significant reduction of serum lipid levels, attenuated hepatic steatosis, and decreased lipid excretion than the control mice. microarray data showed that BM-TTP deficiency modulate liver genes involved in lipid metabolism and inflammatory response. RT-qPCR was used to confirm the results in several genes from microarray data. BM-TTP deficiency did not further accelerate atherorogenesis process in LDLR−/− mice after a Western diet feeding. TTP promoted VLDL to drive proinflammatory mechanism into proatherogenic mechanism.
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| Overall design |
Two-condition, one-color experiment. LDLR knockout mice were transplanted with wild type or TTP knockout bone marrow cells and gene expression was performed on RNA isolated from liver. 4 biological replicates per each experimental group.
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| Contributor(s) |
Altomare D, Saaoud F, Fan D |
| Citation(s) |
32591281 |
| Submission date |
Feb 13, 2019 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Diego Altomare |
| Organization name |
University of South Carolina
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| Department |
Department of Drug Discovery and Biomedical Sciences
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| Lab |
Functional Genomics Core
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| Street address |
715 Sumter Street, Room 617
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| City |
Columbia |
| State/province |
SC |
| ZIP/Postal code |
29208 |
| Country |
USA |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA522059 |
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