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| Status |
Public on May 09, 2019 |
| Title |
Pro-angiogenic transcriptome of zebrafish endothelial cells |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
To identify previously unknown determinants of endothelial cell sprouting, we defined and exploited a pharmacological strategy for the manipulation of angiogenic cell behavior in vivo. Whereas high vascular endothelial growth factor receptor (Vegfr) signaling is known to promote tip cell (TC) specification, activation of the Notch receptor via its ligand Delta-like 4 (Dll4) represses the TC phenotype to promote stalk cell (SC) fate. Conversely, suppression of Notch activity upon antagonistic interaction with its ligand Jagged1 pro- motes TC formation. Hence, specification of TCs involves tight spatiotemporal control of Vegfr/Notch signaling.
Consequently, we hypothesized that the pharmacological manipulation of Vegfr/Notch signaling selectively during zebrafish intersegmental vessel (ISV) angiogenesis would enable the precise control of angiogenic EC behavior and sprouting- associated gene expression in vivo. For more information, see Herbert et al., 2012, PMID 22921365.
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| Overall design |
Zebrafish embryos were treated with DMSO (Control), DAPT (hypersprouting condition), SU5416(inhibition of sprouting) or both from 22hpf to 30hpf.At 30hpf, cells were dissociated and endothelial cells were isiolated for further RNA extraction
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| Contributor(s) |
Herbert SP, Stainier DR |
| Citation(s) |
22921365 |
| Submission date |
May 08, 2019 |
| Last update date |
May 11, 2019 |
| Contact name |
Shane P Herbert |
| E-mail(s) |
shane.herbert@manchester.ac.uk
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| Organization name |
University of Manchester
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| Department |
Faculty of Biology, Medicine and Health
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| Lab |
Division of Developmental Biology and Medicine
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| Street address |
Michael Smith Building, OXford Road
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| City |
Manchester |
| ZIP/Postal code |
M13 9PT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6457 |
Agilent-019161 D. rerio (Zebrafish) Oligo Microarray (V2) G2519F (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA541913 |
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