|
| Status |
Public on Nov 23, 2022 |
| Title |
Mineralocorticoid Receptor Antagonists and Glucocorticoids Differentially Affect Skeletal Muscle Inflammation and Pathology in Muscular Dystrophy |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the RNA Sequencing of myleoid cells isolated from 4.5-week-old mdx mice that were administered spironolactone, prednisolone, or vehicle for 7 days. We find that both spironolactone and prednisolone downregulated expression of genes involved in inflammation and fibrosis, while prednisolone further perturbed cell cycle genes.
|
| |
|
| Overall design |
Single cell suspensions were prepared from quadriceps from 4.5-week-old mdx mice treated with spironolactone, prednisolone, or vehicle for 7 days. Cells from 3 pairs of mdx quadriceps were pooled for each biological replicate. Myeloid cells were isolated and RNA was extracted from myeloid cells.
|
| |
|
| Contributor(s) |
Howard ZM, Gomatam C, Rabolli CP, Lowe J, Bansal SS, Accornero F, Rafael-Fortney JA |
| Citation(s) |
36040807 |
| Submission date |
Feb 27, 2022 |
| Last update date |
Nov 24, 2022 |
| Contact name |
Jill Rafael-Fortney |
| Organization name |
The Ohio State Universtiy
|
| Department |
Physiology and Cell Biology
|
| Street address |
370 W. 9th Avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA810878 |
Less...
More...