|
| Status |
Public on Nov 23, 2022 |
| Title |
myeloid cells, Suc3 |
| Sample type |
SRA |
| |
|
| Source name |
Quadriceps
|
| Organism |
Mus musculus |
| Characteristics |
strain: mdx
tissue: Quadriceps
cell type: myeloid cells
age: 4.5 weeks
treatment: vehicle
|
| Treatment protocol |
Mice were treated with spironolactone, prednisolone, or vehicle for 7 days prior to extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a NucleoSpin RNA XS kit (Takara, 740902)
DNBseq preparation method (BGI)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Data processing |
Sequencing alignment, hisat2 v2.2.1
read count matrix formation, htseq 0.11.3
differential anaysis, DEseq2 v1.32.0
Genome_build: mm39
Supplementary_files_format_and_content: count matrix (.txt)
Supplementary_files_format_and_content: DESeq2 analysis (.csv). DESeq2 analaysis was carried out comparing all 3 groups. Nomenclature example: 'Prd_vs_Veh.csv' compares Prednisolone vs Vehicle conditions. 'Suc' stands for 'Sucrose' which is the Vehicle
|
| |
|
| Submission date |
Feb 27, 2022 |
| Last update date |
Nov 23, 2022 |
| Contact name |
Jill Rafael-Fortney |
| Organization name |
The Ohio State Universtiy
|
| Department |
Physiology and Cell Biology
|
| Street address |
370 W. 9th Avenue
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL28457 |
| Series (1) |
| GSE197553 |
Mineralocorticoid Receptor Antagonists and Glucocorticoids Differentially Affect Skeletal Muscle Inflammation and Pathology in Muscular Dystrophy |
|
| Relations |
| BioSample |
SAMN26287687 |
| SRA |
SRX14306157 |
