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| Status |
Public on Feb 26, 2024 |
| Title |
Gene expression profile at single cell levels of the cells from the controls and tau P251L Drosophila brain. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Tau protein has been implicated in the pathology of Alzheimer's disease and related disorders. We CRISPR engineered an endogenous model of tauopathy by knocking in a pathogenic human tau mutation, tau P301L, in Drosophila, tau P251L KI. We used single cell RNA sequencing to identify the differentially expressed genes or pathways.
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| Overall design |
Control and tau P251L KI brains were dissociated, cells were isolated and analyzed using scRNAseq.
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| Contributor(s) |
Bukhari H, Feany MB |
| Citation(s) |
38352559 |
| Submission date |
Jan 20, 2023 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Hassan Bukhari |
| E-mail(s) |
shbukhari@bwh.harvard.edu
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| Phone |
2175520802
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| Organization name |
Brigham and Womens hospital
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| Department |
Pathology
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| Lab |
Feany lab
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| Street address |
77, Avenue Louise Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA925848 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223345_RAW.tar |
727.0 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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