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| Status |
Public on Oct 17, 2023 |
| Title |
Eosinophils promote lung emphysema via CTSL |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Patients with chronic obstructive pulmonary disease (COPD) having higher blood eosinophil levels exhibit worse lung function and more severe emphysema, implying the potential role of eosinophils in emphysema development. However, the specific mechanism underlying eosinophil-mediated emphysema development is not fully elucidated. In this study, single-cell RNA sequencing was used to identify eosinophil subgroups in mouse models of asthma and emphysema and analyze their functions. Analysis of the accumulated eosinophils revealed differential transcriptomes between the mouse lungs of elastase-induced emphysema and ovalbumin-induced asthma., Eosinophil depletion alleviated elastase-induced emphysema. Notably, eosinophil-derived cathepsin L (CTSL) degraded the extracellular matrix (ECM), causing emphysema in the pulmonary tissue. Eosinophils were positively correlated with serum CTSL levels, which were increased in patients with emphysema than in those without emphysema. Collectively, these results suggest that CTSL expression in eosinophils plays an important role in ECM degradation and remodeling and is related to emphysema in patients with COPD. Therefore, eosinophil-derived CTSL may serve as a potential therapeutic target for patients with emphysema.
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| Overall design |
13 sample from lung tissue
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| Contributor(s) |
Xu X |
| Citation(s) |
37816708 |
| Submission date |
Jul 19, 2023 |
| Last update date |
Oct 17, 2023 |
| Contact name |
1 1 |
| E-mail(s) |
xuxia@ccmu.edu.cn
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| Organization name |
1
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| Street address |
1
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| City |
1 |
| ZIP/Postal code |
1 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA996546 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE237749_barcodes.tsv.gz |
191.7 Kb |
(ftp)(http) |
TSV |
| GSE237749_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSE237749_matrix.mtx.gz |
280.6 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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