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| Status |
Public on Oct 17, 2023 |
| Title |
Con3 |
| Sample type |
SRA |
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| Source name |
lung
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| Organism |
Mus musculus |
| Characteristics |
tissue: lung
disease state: Con
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume BSA. Then Cells were added to each channel. After generation of GEMs, reverse transcription reactions were engaged barcoded full-length cDNA followed by the disruption of emulsions using the recovery agent and cDNA clean up with DynaBeads Myone Silane Beads (Thermo Fisher Scientific). Subsequently, the amplified cDNA was fragmented, end-repaired, A-tailed, and index adaptor ligated and library amplification.
RNA libraries were prepared with Chromium Single cell 3’ Reagent v3 Kits according to the manufacturer’s protocol.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
one sample from lung tissue
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| Data processing |
The Cell Ranger software pipeline provided by 10×Genomics was used to demultiplex cellular barcodes, map reads to the genome and transcriptome using the STAR aligner, and down-sample reads as required to generate normalized aggregate data across samples, producing a matrix of gene counts versus cells
Assembly: mm10
Supplementary files format and content: Matrix table with raw gene counts for every gene and every cell
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| Submission date |
Jul 19, 2023 |
| Last update date |
Oct 17, 2023 |
| Contact name |
1 1 |
| E-mail(s) |
xuxia@ccmu.edu.cn
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| Organization name |
1
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| Street address |
1
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| City |
1 |
| ZIP/Postal code |
1 |
| Country |
China |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE237749 |
Eosinophils promote lung emphysema via CTSL |
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| Relations |
| BioSample |
SAMN36624347 |
| SRA |
SRX21084077 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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