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| Status |
Public on May 14, 2024 |
| Title |
A dynamic subpopulation of CRISPR-Cas overexpressers allows Streptococcus pyogenes to rapidly respond to phage [RNA-seq] |
| Organism |
Streptococcus pyogenes |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cas9 is a transcriptional autoregulator in many strains of S. pyogenes. A natural single guide RNA (tracr-L) directs Cas9 to bind to and repress its promoter. In this study, we aimed to determine whether tracr-L, crRNAs, or other noncanonical guide RNAs direct Cas9 to bind to other regions of the genome and potentially regulate the expression of additional genes.
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| Overall design |
To determine whether CRISPR-Cas overexpression or loss leads to changes in gene expression, we generated strains of S. pyogenes that overexpress (∆tracr-L) or lack (∆CRISPR-Cas) the CRISPR-Cas system and performed RNA-Seq along with the WT strain.
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| Contributor(s) |
Stoltzfus MJ, Workman RE, Keith NC, Modell JW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 10, 2024 |
| Last update date |
May 15, 2024 |
| Contact name |
Joshua Modell |
| Organization name |
Johns Hopkins University School of Medicine
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| Street address |
725 N Wolfe St, PCTB 614
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL28678 |
Illumina NovaSeq 6000 (Streptococcus pyogenes) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA1098752 |
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