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| Status |
Public on May 14, 2024 |
| Title |
SF370_DtrL_stat_biolrep2 |
| Sample type |
SRA |
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| Source name |
SF370
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| Organism |
Streptococcus pyogenes |
| Characteristics |
strain: SF370
genotype: {delta}tracr-L
growth phase: stationary
molecule subtype: rRNA-depleted total RNA
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| Extracted molecule |
total RNA |
| Extraction protocol |
After cell lysis, RNA was extracted with the Direct-zol™ RNA Miniprep Kit (Zymo Research) and treated with Promega RQ1 DNase (M6101) to digest contaminating DNA.
Multiplexed libraries were prepared with NEBNext® rRNA Depletion Kit (Bacteria) (E7850L) and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (E7760S) using 450 ng total RNA input. PCR amplification was performed for 11 rounds with NEBNext® Multiplex Oligos for Illumina®.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Transcripts were quantified with Salmon (v1.8.0)
Assembly: AE004092
Supplementary files format and content: Tab-delimited file containing feature length, TPM, and total reads for each gene feature
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| Submission date |
Apr 10, 2024 |
| Last update date |
May 14, 2024 |
| Contact name |
Joshua Modell |
| Organization name |
Johns Hopkins University School of Medicine
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| Street address |
725 N Wolfe St, PCTB 614
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platform ID |
GPL28678 |
| Series (1) |
| GSE263699 |
A dynamic subpopulation of CRISPR-Cas overexpressers allows Streptococcus pyogenes to rapidly respond to phage [RNA-seq] |
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| Relations |
| BioSample |
SAMN40921093 |
| SRA |
SRX24216320 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM8196765_JW3079_stat2.sf.gz |
30.5 Kb |
(ftp)(http) |
SF |
SRA Run Selector |
| Raw data are available in SRA |
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