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| Status |
Public on May 22, 2012 |
| Title |
Cellular aging can be monitored by DNA-methylation changes at specific CpG sites |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
|
| Summary |
Long-term culture associated changes need to be considered for quality control of cell preparations – especially in cellular therapy. Here we describe a simple method to track cellular aging based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic signature can be used as a biomarker for various cell types to predict the state of cellular aging with regard to the number of passages or days of in vitro culture.
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| |
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| Overall design |
8 samples of human dermal fibroblasts. 4 samples of mesenchymal stem cells (MSC) from human adipose tissue.
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| |
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| Contributor(s) |
Koch CM, Joussen S, Schellenberg A, Lin Q, Zenke M, Wagner W |
| Citation(s) |
22221451 |
| Submission date |
Jun 01, 2011 |
| Last update date |
Jan 02, 2015 |
| Contact name |
Wolfgang Wagner |
| E-mail(s) |
wwagner@ukaachen.de
|
| Phone |
+49 241 8088611
|
| Organization name |
RWTH Aachen University
|
| Department |
Helmholtz Institute for Biomedical Engineering
|
| Lab |
Stem Cell Biology and Cellular Engineering
|
| Street address |
Pauwelsstrasse 20
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL8490 |
Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA141137 |
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