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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 15, 2012 |
| Title |
DNA methylation profiling of embryonic stem cell differentiation into the three germ layers [MeDIP analysis] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Embryogenesis is tightly regulated by multiple levels of epigenetic systems such as DNA methylation, histone modification, and chromatin remodeling. DNA methylation patterns are erased in primordial germ cells and in the interval immediately following fertilization. Subsequent reprogramming occurs by de novo methylation and demethylation. Variance of DNA methylation patterns between different cell types is not well understood. Here, using methylated DNA immunoprecipitation and tiling array technology, we have comprehensively analysed DNA methylation patterns at proximal promoter regions in mouse embryonic stem (ES) cells, ES cell-derived early germ layers (ectoderm, endoderm and mesoderm) and four adult tissues (brain, liver, skeletal muscle and sperm). Most of the methylated regions in the three germ layers and in the three adult somatic tissues are shared in common. This commonly methylated gene set is enriched in germ cell associated genes that are generally transcriptionally inactive in somatic cells. We also compared DNA methylation patterns with global mapping of histone H3 lysine 4/27 trimethylation, and found that gain of DNA methylation correlates with loss of histone H3 lysine 4 trimethylation. Taken together, our findings indicate that differentiation from ES cells to the three germ layers is accompanied by an increase in the number of commonly methylated DNA regions and that these tissue-specific alterations are present for only a small number of genes. Our findings indicate that DNA methylation at the proximal promoter regions of commonly methylated genes act as an irreversible mark which fixes somatic lineage by repressing transcription of germ cell specific genes.
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| Overall design |
Using the MeDIP on chip protocol, we immunoprecipitated methylated DNA from R1 ES cell, SK7 cell, as well as SK7 derived-Ectoderm, - Endoderm, -Paraxial mesoderm, brain, liver, skeletal muscle, and sperm, and hybridized to a genome tiling array. The three germ layer lineages from ES cells were confirmed by the expression of specific marker genes in each germ layer (see related expression analysis).
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| Contributor(s) |
Isagawa T, Nagae G, Shiraki N, Fujita T, Sato N, Ishikawa S, Kume S, Aburatani H |
| Citation(s) |
22016810 |
| Submission date |
Sep 13, 2011 |
| Last update date |
Oct 14, 2014 |
| Contact name |
Takayuki Isagawa |
| E-mail(s) |
isagawa@genome.rcast.u-tokyo.ac.jp
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| Organization name |
The University of Tokyo
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| Department |
RCAST
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| Lab |
Genome Science
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| Street address |
4-6-1 Komaba, Meguro-ku
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| City |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
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| Platforms (1) |
| GPL5811 |
[Mm_PromPR] Affymetrix Mouse Promoter 1.0R Array |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE32082 |
DNA methylation profiling of embryonic stem cell differentiation into the three germ layers |
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| Relations |
| BioProject |
PRJNA154933 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32080_RAW.tar |
1.1 Gb |
(http)(custom) |
TAR (of CEL) |
| GSE32080_brain_v36_pvalue.bar.txt.gz |
25.8 Mb |
(ftp)(http) |
TXT |
| GSE32080_liver_v36_pvalue.bar.txt.gz |
25.8 Mb |
(ftp)(http) |
TXT |
| GSE32080_mESC_R1_v36_pvalue.bar.txt.gz |
28.2 Mb |
(ftp)(http) |
TXT |
| GSE32080_mESC_v36_pvalue.bar.txt.gz |
25.0 Mb |
(ftp)(http) |
TXT |
| GSE32080_mEct_v36_pvalue.bar.txt.gz |
25.1 Mb |
(ftp)(http) |
TXT |
| GSE32080_mEnd_v36_pvalue.bar.txt.gz |
25.4 Mb |
(ftp)(http) |
TXT |
| GSE32080_mPMD_v36_pvalue.bar.txt.gz |
25.4 Mb |
(ftp)(http) |
TXT |
| GSE32080_skmuscle_v36_pvalue.bar.txt.gz |
26.0 Mb |
(ftp)(http) |
TXT |
| GSE32080_sperm_v36_pvalue.bar.txt.gz |
25.9 Mb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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