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| Status |
Public on Oct 05, 2011 |
| Title |
Genome-wide analysis of gene expression of Th1 and Tfh LCMV-specific Smarta CD4 T cell populations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, we sought to distinguish phenotypically and functionally distinct virus-specific effector CD4 T cell subsets that form during acute lymphocytic choriomeningitis virus (LCMV) infection and to examine their ability to develop into memory T cells. To further characterize the effector subsets and identify genetic pathways and transcription factors involved in their differentiation, we performed genome-wide gene expression profiling of the three day 8 effector cell populations: (1) PSGL1hi Ly6Chi, (2) PSGL1hi Ly6Clo and (3) PSGL1lo Ly6Clo Smarta CD4 T cells along with (4) day 60 memory PSGL1hi and (5) naïve Smarta CD4 T cells using Illumina BeadChips. Results of this microarray confirmed the validity of our phenotyping by flow cytometry in many ways and showed distinct gene signatures for the three effector subsets.
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| Overall design |
DNA microarray analysis was performed on two or three independent samples of five different Smarta CD4 T cell populations: (1) naïSGL1lo, (3) day 8 PSGL1hi Ly6Clo, (4) day 8 PSGL1hi Ly6Clo and (5) day 60 PSGL1hi memory cells.
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| Contributor(s) |
Marshall H, Kaech S, Meng H, Kleinstein S |
| Citation(s) |
22018471 |
| Submission date |
Oct 04, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Hailong Meng |
| Organization name |
Yale University
|
| Street address |
300 George Str
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06525 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA147183 |
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