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| Status |
Public on Nov 08, 2006 |
| Title |
Temporal expression profile of CHRF-288 cell line after phorbol ester stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of phorbol-ester-induced Mk differentiation of the megakaryoblastic CHRF-288-11 (CHRF) cell line – a model system for investigating megakaryopoiesis. The goals of this study were to (1) verify the megakaryocytic nature of the CHRF cell line at the transcriptional level, and (2) extract novel insights into the key facets of Mk maturation including polyploidization, proplatelet formation, and apoptosis.
Keywords: time course
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| Overall design |
CHRF-288 cells were cultured in the presence of 10 ng/mL phorbol ester (PMA) or equivalent volume of DMSO solvent (0.02%). Unstimulated control cells from time zero (exponentially growing CHRF cells) were also analyzed. For PMA treated legs, only the adherent cells were included in the transcriptional analysis. Two biological replicate experiments were analyzed and approximately one-half of the samples with each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.
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| Contributor(s) |
Fuhrken PG, Miller WM, Papoutsakis ET |
| Citation(s) |
17309828 |
| Submission date |
Dec 15, 2005 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Eleftherios Terry Papoutsakis |
| E-mail(s) |
papoutsakis@dbi.udel.edu
|
| Organization name |
University of Delaware
|
| Department |
Chemical Engineering
|
| Street address |
15 Innovation Way
|
| City |
Newark |
| State/province |
DE |
| ZIP/Postal code |
19711 |
| Country |
USA |
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| Platforms (1) |
| GPL887 |
Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version) |
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| Samples (41)
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| This SubSeries is part of SuperSeries: |
| GSE8914 |
CHRF-288-11 and primary human megakaryocytic cell cultures provide novel insights into lineage-specific differentiation |
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| Relations |
| BioProject |
PRJNA104985 |
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