 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 17, 2012 |
| Title |
Expression data from IRE1a or XBP1 deficient mouse liver |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
IRE1a and XBP1 are key regulators of the unfolded protein response (UPR). XBP1 ablation causes profound hypolipidemia in mice, and triggers feedback activation of its upstream enzyme IRE1a, instigating regulated IRE1-dependent decay (RIDD), an mRNA degradation mechanism dependent on IRE1a's endoribonuclease activity. Comprehensive microarray analysis of XBP1 and/or IRE1a deficient liver identified genes involved in lipogenesis and lipoprotein metabolism as RIDD substrates, which might contribute to the suppression of plasma lipid levels by activated IRE1a.
|
| |
|
| Overall design |
To identify RIDD substrate mRNAs and direct XBP1 targets in the liver, we performed a comprehensive comparative microarray analysis of three groups of RNA samples: WT and XBP1 deficient mice, WT and IRE1a deficient mice untreated or injected with tunicamycin, and XBP1 deficient mice injected with luciferase or IRE1a siRNA.
|
| |
|
| Contributor(s) |
Lee A, So J, Fitzgerald K, Iwawaki T, Glimcher LH |
| Citation(s) |
23040070 |
| Submission date |
Aug 31, 2012 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Ann-Hwee Lee |
| E-mail(s) |
ahlee@hsph.harvard.edu
|
| Organization name |
Harvard School of public health
|
| Street address |
651 Huntington Ave, FXB/205
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
|
| Samples (31)
|
| GSM995450 |
WT, littermate of Xbp1 ko, biological rep1 |
| GSM995451 |
WT, littermate of Xbp1 ko, biological rep2 |
| GSM995452 |
WT, littermate of Xbp1 ko, biological rep3 |
| GSM995453 |
WT, littermate of Xbp1 ko, biological rep4 |
| GSM995454 |
Xbp1 ko, biological rep1 |
| GSM995455 |
Xbp1 ko, biological rep2 |
| GSM995456 |
Xbp1 ko, biological rep3 |
| GSM995457 |
Xbp1 ko, biological rep4 |
| GSM995458 |
Xbp1 ko, luciferase siRNA, biological rep1 |
| GSM995459 |
Xbp1 ko, luciferase siRNA, biological rep2 |
| GSM995460 |
Xbp1 ko, luciferase siRNA, biological rep3 |
| GSM995461 |
Xbp1 ko, luciferase siRNA, biological rep4 |
| GSM995462 |
Xbp1 ko, IRE1 siRNA, biological rep1 |
| GSM995463 |
Xbp1 ko, IRE1 siRNA, biological rep2 |
| GSM995464 |
Xbp1 ko, IRE1 siRNA, biological rep3 |
| GSM995465 |
Xbp1 ko, IRE1 siRNA, biological rep4 |
| GSM995466 |
WT, littermate of Ire1 ko, biological rep1 |
| GSM995467 |
WT, littermate of Ire1 ko, biological rep2 |
| GSM995468 |
WT, littermate of Ire1 ko, biological rep3 |
| GSM995469 |
WT, littermate of Ire1 ko, biological rep4 |
| GSM995470 |
WT, tunicamycin, biological rep1 |
| GSM995471 |
WT, tunicamycin, biological rep2 |
| GSM995472 |
WT, tunicamycin, biological rep3 |
| GSM995473 |
WT, tunicamycin, biological rep4 |
| GSM995474 |
Ire1 ko, biological rep1 |
| GSM995475 |
Ire1 ko, biological rep2 |
| GSM995476 |
Ire1 ko, biological rep3 |
| GSM995477 |
Ire1 ko, tunicamycin, biological rep1 |
| GSM995478 |
Ire1 ko, tunicamycin, biological rep2 |
| GSM995479 |
Ire1 ko, tunicamycin, biological rep3 |
| GSM995480 |
Ire1 ko, tunicamycin, biological rep4 |
|
| Relations |
| BioProject |
PRJNA174429 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40515_RAW.tar |
58.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...