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| Status |
Public on Jan 29, 2013 |
| Title |
CD109 plays a role in osteoclastogenesis. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The primary aim of this project was to identify novel factors, in particular the cell-surface protein CD109, which regulate osteoclastogenesis. Microarray analysis was performed comparing two pre-osteoclast cell lines generated from the RAW 264.7 osteoclast cell line: one that has the capacity to fuse forming large multinucleated cells and one that does not fuse. It was found that CD109 was up-regulated by > 17-fold in the osteoclast forming cell line when compared to the cell line that does not fuse.
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| Overall design |
H10 (osteoclastogenic cell line) and C8 (non osteoclastogenic cell line) cells were plated for two days in 60 mm tissue culture dishes at a density of 0.5 x 10^6 in a culture medium consisting of 8 ml of Dulbecco's Modified Eagle Medium (DMEM, Life Technologies, Grand Island, NY, USA) supplemented with 10% Fetal Bovine Serum (FBS) and 10% antibiotics (164 IU/mL of penicillin G, 50 mg/ml of gentamicin, and 0.25 mg/ml of fungizone) as well as purified recombinant RANKL (60ng/ml). Following two days of culture, total RNA was extracted from the H10 and C8 cells (Qiagen RNeasy Minikit, Germantown, MD, USA) and the concentration of extracted RNA was measured (using a nanodrop method). Experiment was done in triplicates
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| Contributor(s) |
Inger M, Wang Y, Tenenbaum H, Glogauer M |
| Citation(s) |
23593435 |
| Submission date |
Jan 28, 2013 |
| Last update date |
Sep 17, 2020 |
| Contact name |
Maayan Inger |
| E-mail(s) |
maayan.barashi@mail.utoronto.ca
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| Organization name |
University of Toronto
|
| Street address |
Fitzgerald Building 150 College Street, Room 221
|
| City |
Toroto |
| State/province |
ON |
| ZIP/Postal code |
M5S 3E2 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM1071626 |
Osteoclastogenic cell line_stimulated RANKL_2 days_rep1 |
| GSM1071627 |
Osteoclastogenic cell line_stimulated RANKL_2 days_rep2 |
| GSM1071628 |
Osteoclastogenic cell line_stimulated RANKL_2 days_rep3 |
| GSM1071629 |
Non Osteoclastogenic cell line_stimulated RANKL_2 days_rep1 |
| GSM1071630 |
Non Osteoclastogenic cell line_stimulated RANKL_2 days_rep2 |
| GSM1071631 |
Non Osteoclastogenic cell line_stimulated RANKL_2 days_rep3 |
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| Relations |
| BioProject |
PRJNA187486 |
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