|
| Status |
Public on Mar 28, 2013 |
| Title |
Diet specific expression profiles_MA1 |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of wildtype (N2) C. elegans fed different diets: E. coli OP50, E. coli HT115 and Comamonas DA1877
Dietary composition has major effects on physiology. Here we show that developmental rate, reproduction and lifespan are altered in C. elegans fed Comamonas DA1877 relative to those fed a standard E. coli OP50 diet. We identify a set of genes that change in expression in response to this diet, and use the promoter of one of these (acdh-1) as a dietary sensor. Remarkably, the effects on transcription and development occur even when Comamonas DA1877 is diluted with another diet, suggesting that Comamonas DA1877 generates a signal that is sensed by the nematode. Surprisingly, the developmental effect is independent from TOR and insulin signaling. Rather, Comamonas DA1877 affects cyclic gene expression during molting, likely through the nuclear hormone receptor NHR-23. Altogether, our findings indicate that different bacteria elicit various responses via distinct mechanisms, which has implications for diseases such as obesity and the interactions between the human microbiome and intestinal cells.
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| |
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| Overall design |
3 biological replicates for each condition, OP50 is reference sample
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| |
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| Contributor(s) |
MacNeil LT, Watson E, Arda HE, Zhu LJ, Walhout AJ |
| Citation(s) |
23540701 |
| Submission date |
Jan 31, 2013 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Lesley T MacNeil |
| E-mail(s) |
lesley.macneil@umassmed.edu
|
| Organization name |
UMass Medical School
|
| Department |
Program in Systems Biology
|
| Lab |
Walhout
|
| Street address |
368 Plantation St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
|
| Samples (9)
|
| GSM1074941 |
N2 young adult on E. coli HT115 rep 1 |
| GSM1074942 |
N2 young adult on E. coli HT115 rep 2 |
| GSM1074943 |
N2 young adult on E. coli HT115 rep 3 |
| GSM1074944 |
N2 young adult on Comamonas DA1877 rep 1 |
| GSM1074945 |
N2 young adult on Comamonas DA1877 rep 2 |
| GSM1074946 |
N2 young adult on Comamonas DA1877 rep 3 |
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| This SubSeries is part of SuperSeries: |
| GSE43959 |
Diet-Induced Developmental Acceleration Independent of TOR and Insulin in C. elegans |
|
| Relations |
| BioProject |
PRJNA188225 |
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