|
| Status |
Public on Mar 28, 2013 |
| Title |
N2 young adult on E. coli HT115 rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Young adult
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: wild type (N2)
age: Young adult
diet: E. coli HT115
|
| Growth protocol |
Animals grown on NGM plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA prepared with Trizol(life technologies) followed by cleanup with RNeasy columns(Qiagen) as per manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Affymetrix 3' IVT Express Kit from 500ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip C. elegans Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
HT115-3
HTDE3.3
|
| Data processing |
Expression Console software from Affymetrix was used to determine data quality as well as for generation of pivot files using RMA
|
| |
|
| Submission date |
Jan 31, 2013 |
| Last update date |
Mar 28, 2013 |
| Contact name |
Lesley T MacNeil |
| E-mail(s) |
lesley.macneil@umassmed.edu
|
| Organization name |
UMass Medical School
|
| Department |
Program in Systems Biology
|
| Lab |
Walhout
|
| Street address |
368 Plantation St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (2) |
| GSE43954 |
Diet specific expression profiles_MA1 |
| GSE43959 |
Diet-Induced Developmental Acceleration Independent of TOR and Insulin in C. elegans |
|
