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| Status |
Public on Oct 17, 2013 |
| Title |
Krüppel-like factor 3 KO and rescue expression data in murine embyronic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Krüppel-like factor 3 (KLF3) is a transcriptional repressor that has roles in adipogenesis, B-cell maturation and erythropoiesis (for review see Pearson et al., 2012).
We profiled gene expression in murine embryonic fibroblasts (MEFs) where Klf3 had been knocked-out and where the same cell had been rescued with Klf3 using microarrays.
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| Overall design |
Klf3 KO murine embryonic fibroblast cell lines were produced from Klf3 KO mice. These cells were rescued with epitope tagged Klf3 (Klf3-V5) using retroviral delivery (murine stem cell virus). Stable clones were selected uner puromycin seletion and total RNA was taken for array
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| Contributor(s) |
Burdach J, Funnell AP, Artuz CM, Sin MK, Tan LY, Pearson RC, Crossley M |
| Citation(s) |
24106088 |
| Submission date |
Feb 28, 2013 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Jon Burdach |
| Organization name |
University of New South Wales
|
| Department |
BABS
|
| Street address |
UNSW
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA191576 |
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