|
| Status |
Public on Oct 17, 2013 |
| Title |
MEF_Klf3-Rescue_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Cultured Klf3-/- MEF cells, rescued with Klf3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: MEFs
genotype/variation: Klf3-/- rescued with Klf3
|
| Growth protocol |
Cells were grown in DMEM supplemented with 10% FCS and 1X PSG
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from Klf3-/- or Klf3 rescued MEF cells using tri-reagent according to the manufacturer’s instructions (Sigma-Aldrich, St Louis, MO). RNA was subsequently ethanol precipitated and washed with 75% ethanol in DEPC-treated deionized water for further purification.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using the Affymetrix IVT Express kit
|
| |
|
| Hybridization protocol |
RNA was hybridized to Affymetrix Mouse Gene 1.0 ST arrays following manufacturers instructions, by the Ramaciotti Centre for Gene Function, UNSW, Sydney, Australia
|
| Scan protocol |
Arrays were scanned following manufacturers instructions, by the Ramaciotti Centre for Gene Function, UNSW, Sydney, Australia
|
| Data processing |
Microarray data were analyzed using Partek Genomic Suite v6.5 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
|
| |
|
| Submission date |
Feb 28, 2013 |
| Last update date |
Oct 17, 2013 |
| Contact name |
Jon Burdach |
| Organization name |
University of New South Wales
|
| Department |
BABS
|
| Street address |
UNSW
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE44744 |
Krüppel-like factor 3 KO and rescue expression data in murine embyronic fibroblasts |
|
