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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 01, 2015 |
| Title |
Compound mouse mutants of bZIP transcription factors MafG and MafK reveal a regulatory network of non-crystallin genes linled to cataract |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Although majority of the genes linked to pediatric cataract exhibit lens fiber cell-enriched expression, our understanding of gene regulation in these cells is limited to function of just eight transcription factors and largely in the context of crystallins. Here, we identify small Maf transcription factors MafG and MafK as regulators of several non-crystallin human cataract genes in fiber cells and establish their significance to cataract. We applied a bioinformatics tool for cataract gene discovery iSyTE to identify MafG and its co-regulators in the lens, and generated various null-allelic combinations of MafG:MafK mouse mutants for phenotypic and molecular analysis. By age 4-months, MafG-/-:MafK+/- mutants exhibit lens defects that progressively develop into cataract. High-resolution phenotypic characterization of MafG-/-:MafK+/- lens reveals severe defects in fiber cells, while microarrays-based expression profiling identifies 97 differentially regulated genes (DRGs). Integrative analysis of MafG-/-:MafK+/- lens-DRGs with 1) binding-motifs and genomic targets of small Mafs and their regulatory partners, 2) iSyTE lens-expression data, and 3) interactions between DRGs in the String database, unravels a detailed small Maf regulatory network in the lens, several nodes of which are linked to human cataract. This analysis prioritizes 36 highly promising candidates from the original 97 DRGs. Significantly, 8/36 (22%) DRGs are associated with cataracts in human (GSTO1, MGST1, SC4MOL, UCHL1) or mouse (Aldh3a1, Crygf, Hspb1, Pcbd1), suggesting a multifactorial etiology that includes elevation of oxidative stress. These data identify MafG and MafK as new cataract-associated candidates and define their function in regulating largely non-crystallin genes linked to mouse and human cataract.
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| Overall design |
Microarray comparision of lenses from mixed background (129Sv/J, C57BL/6J, and ICR) control (MafG+/-:MafK+/-; no-cataract) and compound (MafG-/-:MafK+/-; cataract) mouse mutants
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| Contributor(s) |
Lachke SA, Motohashi H, Yamamoto M, Anand D, Kakrana A |
| Citation(s) |
25896808, 26185746 |
| Submission date |
Feb 02, 2015 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Deepti Anand |
| E-mail(s) |
anand@udel.edu
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| Organization name |
University of Delaware
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| Department |
Biological Sciences
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| Street address |
351 Wolf Hall, University of Delaware
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| City |
Newark |
| State/province |
Delaware |
| ZIP/Postal code |
19716 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (4)
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| GSM1598872 |
Genes regulation by sMaf (MafG and MafK) in the lens [T1 (Test sample 1)] |
| GSM1598873 |
Genes regulation by sMaf (MafG and MafK) in the lens [T2 (Test sample 2)] |
| GSM1598874 |
Genes regulation by sMaf (MafG and MafK) in the lens [C1 (Control sample 1)] |
| GSM1598875 |
Genes regulation by sMaf (MafG and MafK) in the lens [C2 (Control sample 2)] |
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| Relations |
| BioProject |
PRJNA274227 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65500_MafG_non-normalized.txt.gz |
9.5 Mb |
(ftp)(http) |
TXT |
| GSE65500_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
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