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| Status |
Public on May 25, 2015 |
| Title |
Gene expression signatures in GIST-T1 cells transfected with a miR-335 mimic molecule |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To clarify the effect of miRNAs, we carried out a gene expression microarray analysis using GIST-T1 cells transfected with a miR-335 mimic or a negative control. We found that 1,095 probe sets (853 unique genes) were downregulated (>1.5-fold) by ectopic miR-335 expression.
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| Overall design |
GIST-T1 cells were transfected with a mirVana miR-335 mimic (Ambion) or a mirVana miRNA mimic Negative Control #1 (Ambion). Forty-eight hours after transfection, total RNA extraction was carried out, and gene expression signatures were analyzed.
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| Contributor(s) |
Suzuki H, Niinuma T |
| Citation(s) |
26214687 |
| Submission date |
May 11, 2015 |
| Last update date |
Mar 27, 2018 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
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| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
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| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
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| Samples (4)
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| GSM1680259 |
GIST-T1 cells with miR-335 mimic molecule_1 |
| GSM1680260 |
GIST-T1 cells with miR-335 mimic molecule_2 |
| GSM1680261 |
GIST-T1 cells with control molecule_3 |
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| This SubSeries is part of SuperSeries: |
| GSE68743 |
Gene expression signatures in GIST-T1 cells transfected with miR-34a and miR-335 mimic molecules |
|
| Relations |
| BioProject |
PRJNA283818 |
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