|
| Status |
Public on May 25, 2015 |
| Title |
GIST-T1 cells with miR-335 mimic molecule_2 |
| Sample type |
RNA |
| |
|
| Source name |
GIST-T1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GIST-T1
transfection: miR-335 mimic
|
| Treatment protocol |
GIST-T1 cells (3.0E+6 cells) were transfected with 100 pmol of a miR-335 mirVana miRNA mimics (Ambion) or a mirVana miRNA mimic Negative Control #1 (Ambion) using a Cell Line Nucleofector kit L (Lonza) and a Nucleofector device (Lonza) according to the manufacturer’s instructions. Forty-eight hours after transfection, total RNA extraction was carried out.
|
| Growth protocol |
GIST-T1 cells were cultured in Dulbecco's Modified Eagle's Medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37 ºC in a humidified incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a TRIZOL reagent (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
gene expression in GIST-T1 cells transfected with miR-335 mimic molecule (replicate 2)
miR-34a_mimic_2
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
|
| |
|
| Submission date |
May 11, 2015 |
| Last update date |
May 25, 2015 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16699 |
| Series (2) |
| GSE68742 |
Gene expression signatures in GIST-T1 cells transfected with a miR-335 mimic molecule |
| GSE68743 |
Gene expression signatures in GIST-T1 cells transfected with miR-34a and miR-335 mimic molecules |
|
