|
| Status |
Public on Nov 01, 2015 |
| Title |
Histone chaperone CAF-1 is essential for retrotransposon silencing by mediating histone H4K20me3 deposition in mouse preimplantation embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Retrotransposons are widely spread in the mammalian genome and are usually silenced during development to avoid transposition-inducing mutations. But how they are repressed in embryos shortly before implantation remain to be identified, since the genome at this stage is globally hypomethylated. Here we show a histone chaperon, CAF-1, is responsible for retrotransposon silencing at the morula-blastocyst stages by depositing histone H4 lysine 20 trimethylation (H4K20me3). Knockdown of CAF-1 with a specific siRNA resulted in derepression of LINE-1, SINE-B2 and IAP associated with the decreased H4K20me3 level, and arrested embryonic development at the morula stage. The identical results were obtained with siRNAs against Suv420h1/2, H4K20 methyltransferases. Treatment with reverse transcriptase inhibitors rescued at least a part of these embryos. Thus, CAF-1 ensures the genomic integrity of preimplantation embryos by establishing repressive histone marks in the multiple retrotransposon classes.
|
| |
|
| Overall design |
Comparative gene expression analyses using P150 knockdown (P150KD) embryos at morula stage were performed by microarray. P150KD embryos were produced with the injection of P150 siRNA into 1-cell embryos. As controls, siControl embryos were produced by the injection of control siRNA. These embryos were subjected to gene expression microarray.
|
| |
|
| Contributor(s) |
Hatanaka Y, Inoue K, Ogura A |
| Citation(s) |
26546670 |
| Submission date |
May 27, 2015 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Kimiko Inoue |
| E-mail(s) |
kimiko.inoue@riken.jp
|
| Organization name |
BRC, RIKEN
|
| Department |
Bioresource Engineering Division
|
| Street address |
3-1-1 Koyadai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-0074 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA285041 |
Less...
More...