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| Status |
Public on Aug 31, 2016 |
| Title |
An Investigation of Circulating microRNA Profile in Clinical Cases of Subarachnoid Hemorrhages of Hong Kong |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
circulating microRNA analysis in Subarachnoid hemorrhages (SAH) patients and Heathy controls
circulating microRNA profiling has been shown for biomarker discovery for early disease detection in serum, which is stable and accessible. we describe our progress and propose a workflow to investigate and directly compare the circulating microRNA profiles of SAH patients and healthy individuals, in order to characterize potential biomarkers for SAH. Then we verified the top microRNA by quantitative PCR. Informatics analysis of KEGG provided important mechanistic insight to their potential involved pathways. Finally we demonstrated a possible workflow and initial results which indicated that circulating miRNAs can be potential biomarkers of SAH.
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| Overall design |
We analyzed circulating miRNA from healthy controls (n=20) and SAH patients (n=20) using the Affymetrix miRNA 3.0 platform. Array data was processed by Affymetrix ConsoleTool. No techinical replicates were performed.
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| Contributor(s) |
Wong GK |
| Citation(s) |
26675167 |
| Submission date |
Aug 25, 2015 |
| Last update date |
Jan 08, 2021 |
| Contact name |
Kwok Chu Wong |
| E-mail(s) |
georgewong@surgery.cuhk.edu.hk
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| Organization name |
The Chinese University of Hong Kong
|
| Department |
Surgery
|
| Street address |
Prince of Wales Hospital
|
| City |
Shatin |
| ZIP/Postal code |
00852 |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA293801 |
