|
| Status |
Public on Oct 30, 2015 |
| Title |
Cell cycle-dependent reconfiguration of the DNA (hydroxy) methylome during terminal differentiation of human B cells into plasma cells [expression array] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Molecular mechanisms underlying terminal differentiation of B-cells into plasma cells are major determinants of adaptive immunity but remain only partially understood. Here, we present the transcriptional and epigenomic landscapes of cell subsets arising from activation of human naive B-cells and differentiation into plasmablasts. Cell proliferation of activated B cells was linked to a slight decrease in DNA methylation levels but followed by a committal step in which an S-phase-synchronized differentiation switch was associated with an extensive DNA demethylation and local acquisition of 5-hydroxymethylcytosine at enhancers and genes related to plasma cell identity.
|
| |
|
| Overall design |
Number of samples analyzed: 16 in vitro-derived human B cells, spanning 5 cell populations. No technical replicates.
|
| |
|
| Contributor(s) |
Fest T, Martin-Subero JI, Delaloy C, Rossille D |
| Citation(s) |
26565917 |
| Submission date |
Aug 28, 2015 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Gilles Salbert |
| E-mail(s) |
gilles.salbert@univ-rennes1.fr
|
| Phone |
+33 223 236 625
|
| Organization name |
University of Rennes 1
|
| Department |
Biology
|
| Lab |
UMR6290 CNRS
|
| Street address |
Avenue du Général Leclerc
|
| City |
Rennes |
| ZIP/Postal code |
35042 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
|
| Samples (16)
|
| GSM1863295 |
RNA-P1 subpopulation [BN123_P1] |
| GSM1863296 |
RNA-D4_CFSEhi subpopulation [BN148_D4 CFSEhi] |
| GSM1863297 |
RNA-D4_CFSElo subpopulation [BN148_D4 CFSElo] |
|
| This SubSeries is part of SuperSeries: |
| GSE72498 |
Cell cycle-dependent reconfiguration of the DNA (hydroxy) methylome during terminal differentiation of human B cells into plasma cells |
|
| Relations |
| BioProject |
PRJNA294162 |
Less...
More...