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| Status |
Public on Mar 22, 2017 |
| Title |
Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest-like precursors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, we propose a novel method for inducing neuronal cells by briefly exposing them to small-molecule cocktails in a step-by-step manner. Global gene expression analysis with immunohistochemical staining and calcium flux assays reveal the generation of neurons from mouse embryonic fibroblasts (MEFs). In addition, time-lapse imaging of neural precursor-specific enhancer expression and global gene expression analyses show that the neurons are generated by passing through a neural crest-like precursor stage. Consistent with these results, the neural crest-like cells are able to differentiate into neural crest lineage cells, such as sympathetic neurons, adipocytes, osteocytes, and smooth muscle cells. Therefore, these results indicate that brief exposure to chemical compounds could expand and induce a substantial multipotent cell population without viral transduction.
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| Overall design |
Gene expression of MEFs was measured before and after exposure to small molecular cocktails. MEF_day0: before treatment (ctrl). MEF_day10: after treatment (chem). Experiments were triplicated for each condition.
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| Contributor(s) |
Takayama Y, Wakabayashi T, Kushige H, Saito Y, Shibuya Y, Shibata S, Akamatsu W, Okano H, Kida YS |
| Citation(s) |
28129669 |
| Submission date |
Mar 04, 2016 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Yasuyuki S Kida |
| E-mail(s) |
y-kida@aist.go.jp
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| Organization name |
National Institute of Advanced Industrial Science and Technology (AIST)
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| Street address |
Central 5-41, Higashi 1-1-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8565 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA314320 |
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