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| Status |
Public on Nov 06, 2017 |
| Title |
DNA hypermethylation enhanced telomerase reverse transcriptase expression in human induced pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
During reprogramming into human induced pluripotent stem cell (iPSCs), several stem cell marker genes are induced, such as OCT-4, NANOG, SALL4, and TERT. OCT-4, NANOG, and SALL4 gene expression can be regulated by DNA methylation. Their promoters become hypomethylated in iPSCs during reprogramming, leading to their induced expression. However, epigenetic regulation of the TERT gene remains unclear. In this study, we focused on epigenetic regulation of the human TERT gene and identified a differentially methylated region (DMR) at a distal region in the TERT promoter between human iPSCs and their parental somatic cells. Interestingly, the TERT-DMR was highly methylated in iPSCs, but low-level methylation was observed in their parental somatic cells. Region-specific, methylated-promoter assays showed that the methylated TERT-DMR up-regulated the promoter activity in iPSCs. In addition, Lamin B1 accumulated at the TERT-DMR in iPSCs, but not in their parent somatic cells. These results suggested that the TERT transcription was enhanced by DNA methylation at the TERT-DMR via binding to nuclear lamina during reprogramming. Our findings shed light on a new functional aspect of DNA methylation in gene expression.
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| Overall design |
Bisulphite converted DNA from the samples were hybridized to the Illumina Infinium Human Methylation450K Beadchip
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| Contributor(s) |
Takasawa K, Arai Y, Inoue-Yamazaki M, Toyoda M, Akutsu H, Umezawa A, Nishino K |
| Citation(s) |
29103143, 32210272 |
| Submission date |
Jun 06, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Akihiro Umezawa |
| E-mail(s) |
umezawa@1985.jukuin.keio.ac.jp
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| Phone |
81-3-5494-7047
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| Organization name |
National Center for Child Health and Development
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| Department |
Center for Regenerative Medicine
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| Street address |
2-10-1 Okura
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| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA389403 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99716_RAW.tar |
239.1 Mb |
(http)(custom) |
TAR (of IDAT) |
| GSE99716_signal_intensities.txt.gz |
31.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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