|
| Status |
Public on Apr 01, 2013 |
| Title |
Lung carcinoma patient6 15min central |
| Sample type |
RNA |
| |
|
| Source name |
Lung carcinoma, patient6, 15min, central
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: central lung carcinoma tumor
individual: patient 6
time: 15 min
treatment: cryopreservation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted withTotal RNA Isolation NucleoSpin® RNA II kit (Macherey Nagel) in accordance with the prescribed protocol provided with the kit. Quality control was performed withAgilent 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
500ng of mRNA was amplified into cRNA and biotinylated cRNA using Illumina® TotalPrep™-24 RNA Amplification Kit (Life Technologies).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were processed using log transformation and quantile normalisation with BRBArrayTools
|
| |
|
| Submission date |
Sep 26, 2012 |
| Last update date |
Apr 01, 2013 |
| Contact name |
FLORENCE LE CALVEZ-KELM |
| E-mail(s) |
lecalvez@iarc.fr
|
| Phone |
+33 4 72 73 83 58
|
| Organization name |
International Agency for Research on Cancer
|
| Department |
Genetics
|
| Lab |
Genetic Cancer Susceptibility
|
| Street address |
150 Cours Albert Thomas
|
| City |
LYON |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE41160 |
Impact of delay to cryopreservation on RNA integrity and genome-wide expression profiles in resected tumor samples. |
|
