|
| Status |
Public on Nov 01, 2012 |
| Title |
Histone H3K27me3 ChIP-Seq of CD19 Primary Cells; Histone.DS22585 |
| Sample type |
SRA |
| |
|
| Source name |
flow sorted CD19+ Cells, year 21; Histone.DS22585
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: CD19.RO_01721
sample common name: CD19 Primary Cells
molecule: genomic DNA
disease: None
biomaterial_provider: FHCRC HEIMFELD
biomaterial_type: Primary Cell
cell_type: CD19 Primary Cells
markers: CD19+
donor_id: RO 01679
donor_age: year 21
donor_health_status: NA
donor_sex: Male
donor_ethnicity: Caucasian
passage_if_expanded: NA
experiment_type: Histone H3K27me3
extraction_protocol: http://www.roadmapepigenomics.org/protocols/type/experimental
extraction_protocol_type_of_sonicator: Diagenode Bioruptor
extraction_protocol_sonication_cycles: 20-40 cycles of 0.5 minute on and 0.5 minute off
chip_protocol: http://www.roadmapepigenomics.org/protocolstype/experimental
chip_protocol_chromatin_amount: 60ug
chip_protocol_bead_type: Millipore A/G agarose beads
chip_protocol_bead_amount: 30ul A/G bead slurry
chip_protocol_antibody_amount: 2.4ug
chip_antibody: Histone H3K27me3
chip_antibody_provider: Millipore
chip_antibody_catalog: 07-449
chip_antibody_lot: 1959680
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
sample_term_id: CL_0000236
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Histone H3K27me3 ChIP-Seq
Library name: Histone.DS22585
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.14512
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.6965
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM1027290_UW.CD19_Primary_Cells.H3K27me3.RO_01679.Histone.DS22585.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Histone.DS22585-Histone.DS22585.hg19.level.1.release.9
ANALYSIS TITLE: Mapping of CD19 Primary Cells H3K27me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by H3K27me3 ChIP-Seq on CD19 Primary Cells, Donor RO 01679, were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.16844
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 9
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 22,827,570
FINDPEAKS_SCORE: 0.0349
FINDPEAKS_PERCENTILE: 27
HOTSPOT_SCORE: 0.1319
HOTSPOT_PERCENTILE: 34
IROC_SCORE: 0.772
IROC_PERCENTILE: 24
POISSON_SCORE: 0.3045
POISSON_PERCENTILE: 43
MAXIMUM_REPLICATE_CORRELATION: 0.98
**********************************************************************
ANALYSIS FILE NAME: GSM1027290_UW.CD19_Primary_Cells.H3K27me3.RO_01679.Histone.DS22585.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Histone.DS22585-Histone.DS22585.hg19.level.2.release.9
ANALYSIS TITLE: Raw Signal Density Graphs of CD19 Primary Cells H3K27me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina H3K27me3 ChIP-Seq read mappings from CD19 Primary Cells, Donor RO 01679, were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.16940
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 9
BROWSER_TRACK_NAME: CD19 H3K27me3 79 85
BROWSER_TRACK_DESCRIPTION: UW CD19 Primary Cells Histone H3K27me3 Donor RO 01679 Library Histone.DS22585 EA Release 9
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 22,827,570
FINDPEAKS_SCORE: 0.0349
FINDPEAKS_PERCENTILE: 27
HOTSPOT_SCORE: 0.1319
HOTSPOT_PERCENTILE: 34
IROC_SCORE: 0.772
IROC_PERCENTILE: 24
POISSON_SCORE: 0.3045
POISSON_PERCENTILE: 43
MAXIMUM_REPLICATE_CORRELATION: 0.98
**********************************************************************
|
| |
|
| Submission date |
Oct 29, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Northwest REMC |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
University of Washington
|
| Street address |
-
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE18927 |
University of Washington Human Reference Epigenome Mapping Project |
|
| Relations |
| SRA |
SRX201777 |
| BioSample |
SAMN00254246 |
| Named Annotation |
GSM1027290_UW.CD19_Primary_Cells.H3K27me3.RO_01679.Histone.DS22585.wig.gz |
