|
| Status |
Public on Dec 01, 2013 |
| Title |
Transformed 3T3 cells by mucAB 12_1 |
| Sample type |
RNA |
| |
|
| Source name |
BALB 3T3
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
cell type: 3T3
treatment: transformed 3T3 cells by mucAB
|
| Treatment protocol |
Plasmid pTE40, containing mucAB ligated to the mouse metalothionein promoter, was introduced into 3T3 cells using Dharma FECT (Thermo) and selected for neo resistance. Selected clones were confirmed for the presence of full length mucAB.
|
| Growth protocol |
Cells were cultured with MEM Prime medium (Invitrogen) supplemented with 10% fetus calf serum, with or without neomycin depending on cell type, in a humidified C02 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 100 ng total RNA by GeneChip 3' IVT Express Kit. The procedure was according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 ﾟC on GeneChip Mouse Expression Array Set 430. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
|
| Description |
Gene expression data using Mouse Expression Array 430A
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using default settings by Expression Console ver 1.1.
|
| |
|
| Submission date |
Dec 14, 2012 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Hiroki Sasaki |
| Organization name |
National Cancer Center Reseach Institute
|
| Street address |
Tukiji5-1-1
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE42927 |
Expression data from cultured mouse BALB 3T3 cells containing bacterial plasmid gene mucAB |
|
