|
| Status |
Public on May 31, 2006 |
| Title |
d5 empty vector MOE430A |
| Sample type |
RNA |
| |
|
| Source name |
ES cells (CCE cell line) d5 empty vector
|
| Organism |
Mus musculus |
| Characteristics |
Mouse embrionic stem cells
Gender: male
Strain: 129/Sv
|
| Treatment protocol |
48 hours after transduction cells were re-plated at the density 0.3x10^6 cells per 10 cm dish. Simultaneously, GFP+ cells were FACS purified and used as day 0 time point. Re-plated cells were maintained in the presence of LIF. GFP+ cells from each culture were FACS purified every day for 7 consecutive days to obtain 8 day time course (d0-d7).
|
| Growth protocol |
Cells were maintained in the presence of LIF 1000 unit/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA probes were synthesized using the BioArray High-Yield Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY).
|
| |
|
| Hybridization protocol |
15 micrograms of biotin-labeled cRNA was fragmented for 35min. at 94C in fragmentation buffer (Affymetrix; Santa Clara, CA), and hybridized to Affymetrix MOE 430 A and B oligonucleotide microarrays.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from ES cells transduced with empty vector at d5.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Apr 17, 2006 |
| Last update date |
Apr 18, 2006 |
| Contact name |
Ihor Lemischka |
| Organization name |
Princeton University
|
| Street address |
Washington Rd
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08540 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE4679 |
shRNAi induced differentiation time courses in mouse embrionic stem cells. |
|
