|
| Status |
Public on Jan 24, 2013 |
| Title |
Fibroblast_100 µM kaemferol _24h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HDFa fibroblasts, 100 µM kaemferol, 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HDFa
cell type: normal fibroblasts
treatment: 100 µM kaemferol
treatment duration: 24h
|
| Treatment protocol |
The medium was replaced by fresh medium non-supplemented, containing dimethyl sulfooxide (DMSO) added to a final concentration of 0.05% or 30, 60 or 100 µM kaemferol; 60 or 100 µM daidzein; 30 µM + 30 µM kaemferol/genistein; 30 µM + 30 µM daidzein/genistein concentrations, with 0.05% DMSO. The experimental treatment was carried out for 24 h and 48 h periods.
|
| Growth protocol |
Fibroblasts were cultured from cryo-preserved cells in Dulbecco's Modified Eagle's Medium containing 10% fetal bovine serum and 1% antibiotic/antimycotic solution at 37 oC in a humidified atmosphere of 5% CO2. 10E5 cells were seeded in 6-well plates and grown to a confluence of approximately 80%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the High Pure RNA Isolation Kit in accordance with the prescribed protocol, quantified with the Quant iT RiboGreen Assay Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion TotalPrepAmp Kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
24h100K_2
Replicate 2
|
| Data processing |
The data were normalized using quantile normalization with Illumina GenomeStudio software package (gene expression module version 1.9.0).
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
Jan 24, 2013 |
| Contact name |
Magdalena Gabig-Cimińska |
| E-mail(s) |
m.gabig@biol.ug.edu.pl
|
| Organization name |
University of Gdańsk
|
| Department |
of Molecular Biology
|
| Street address |
Wita Stwosza 59
|
| City |
Gdańsk |
| ZIP/Postal code |
80-308 |
| Country |
Poland |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE43692 |
Genome-wide microarray analysis of normal human fibroblasts in response to kaemferol, daidzein, kaemferol/genistein, and daidzein/genistein |
|
