|
| Status |
Public on Feb 15, 2013 |
| Title |
4184N_CRPC |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP derived xenograft_CRPC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type origin: Cultured LNCaP cells
tissue: LNCaP derived xenograft
harvest time point (along the psa curve): CRPC
|
| Growth protocol |
Cell Culture. Cultured LNCaP cells (derived from the lymph node metastasis of a prostate cancer patient), passage 35–45, were maintained in RPMI 1640 with 5% FCS at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the mirVana™ miRNA Isolation Kit (Ambion AM1560)
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA was used as input for generation of Cyanine-3-labeled cRNA following Agilent’s One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling v6.0.
|
| |
|
| Hybridization protocol |
1650ng labeled cRNA was hybridized on Agilent SurePrint G3 Human GE 4x180K Custom Microarray (Design ID 032034 )
|
| Scan protocol |
Slides were scanned with the Agilent DNA Microarray Scanner at a 3um scan resolution using the one colour GEand 20 bit tiff file settings
|
| Description |
SAMPLE 28
|
| Data processing |
Image files were processed with Agilent Feature Extraction 10.5.1.1 to produce spatially detrended and background subtracted gProcessedSignal intensities
|
| |
|
| Submission date |
Feb 14, 2013 |
| Last update date |
Feb 15, 2013 |
| Contact name |
Shawn Anderson |
| E-mail(s) |
Sanderson@prostatecentre.com
|
| Organization name |
Vancouver Prostate Centre
|
| Lab |
Laboratory for Advanced Genome Analysis
|
| Street address |
2660 Oak Street
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6H3Z6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16604 |
| Series (1) |
| GSE44319 |
The E3 ubiquitin ligase Siah2 contributes to castration-resistant prostate cancer by regulation of androgen receptor transcriptional activity cancer. |
|
