|
| Status |
Public on Nov 15, 2013 |
| Title |
Lin- cells_BCR-ABL_Flt3L_day5_exp2 |
| Sample type |
RNA |
| |
|
| Source name |
Lin- cells, MIG-BCR-ABL+empty MICD8 transduced, Flt3L, day 5, experiment 2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Bone marrow derived Lin- cells
treatment period: 5 day
transduction condition: MIG-BCR-ABL + MICD8
|
| Treatment protocol |
Transduced cells were FACS-sorted and cultured with 100 ng/ml human Flt3L for 5 days.
|
| Growth protocol |
Murine bone marrow Lin- cells were precultured for 24 hours with 100 ng/mL SCF, 10 ng/mL IL-6, and 10 ng/mL IL-3, and transduced with retroviruses by spinoculation on two consecutive days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNAiso Plus reagent (Takara) and further purified using RNeasy Mini Kit (Qiagen) according to the manufacturers' instructions.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled using the Agilent Low Input Quick Amp Labeling Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Slides were hybridized according to the manufacturer's protocol (One-Color Microarray-Based Gene Expression Analysis Protocol, Product # G4140-90040, Version 6.5, May 2010).
|
| Scan protocol |
Slides were scanned with an Agilent DNA Microarray Scanner (model G2505-60502) at 100% in Green channel, with a scan resolution of 3 um.
|
| Data processing |
Data are extracted with Agilent Feature Extraction Software and quantile-normalized.
|
| |
|
| Submission date |
Mar 06, 2013 |
| Last update date |
Nov 16, 2013 |
| Contact name |
Tomohiko Tamura |
| E-mail(s) |
tamurat@yokohama-cu.ac.jp
|
| Organization name |
Yokohama City University
|
| Department |
Department of Immunology
|
| Street address |
3-9 Fukuura, Kanazawa-ku
|
| City |
Yokohama |
| ZIP/Postal code |
236-0004 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE44920 |
Gene epxression profiling in murine bone marrow Lin- cells transduced with BCR-ABL and/or IRF8 followed by Flt3L culture for 5 days |
|
