|
| Status |
Public on Nov 27, 2013 |
| Title |
Fractured Day 2 αSMA-labeled cells rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Tibial periosteum cells from Day 2 fracture after digestion and cell sorting
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: B6;129S6
age: 4.5-5.5 months
genotype: αSMACreERT2/Ai9
n animals: 8
|
| Treatment protocol |
Mice were given 2 doses of tamoxifen (75ug/g) on 2 consecutive days before fracture, or 2 days before collection of control samples.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and biotinylated using the Ambion MessageAmp Premier RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Dissected tissue was digested in PBS containing 0.125% trypsin, 0.1% collagenase A, 0.1% hyaluronadase for 1 h at 37°C with agitation prior to cell sorting. Mice were given 2 doses of tamoxifen, 75ug/g, fractures were performed, then mice were sacrificed two days later.
|
| Data processing |
The data were normalised using quantile normalisation with Illumina GenomeStudio
|
| |
|
| Submission date |
Mar 14, 2013 |
| Last update date |
Nov 27, 2013 |
| Contact name |
Brya Matthews |
| E-mail(s) |
brya.matthews@auckland.ac.nz
|
| Organization name |
University of Auckland
|
| Department |
Molecular Medicine and Pathology
|
| Street address |
85 Park Rd
|
| City |
Grafton |
| State/province |
Auckland |
| ZIP/Postal code |
1023 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE45156 |
Gene expression in alpha SMA Cre-labeled cells in periosteum during early fracture callus formation |
|
