|
| Status |
Public on Sep 08, 2014 |
| Title |
Nanog-GFP OSKM#2 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Poor quality_OSKM iPSC line
|
| Organism |
Mus musculus |
| Characteristics |
iPSc/esc line: Nanog-GFP OSKM#2
iPSc group: Poor quality
|
| Treatment protocol |
No treartment, basal condition
|
| Growth protocol |
Cells were grown in 2i medium withour feeder as described in the manuscript
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Rneasy kit (Qiagen-74106), 300 ng were used for hybridization
|
| Label |
Cy3
|
| Label protocol |
Standard Agilent protocol
|
| |
|
| Hybridization protocol |
Agilent (mouse 8x60k) expression arrays were hybridized according to manufacturer instructions and scanned using an Agilent DNA microarray scanner.
|
| Scan protocol |
Arrays were scanned using an Agilent scanner and the data was extracted using Agilent’s Feature Extraction software.
|
| Description |
252800514386_201207121030_S01_GE1-v5_10_Apr08_1_1.YB1
|
| Data processing |
Arrays were quantile normalized using limma from Bioconductor in R.
|
| |
|
| Submission date |
Mar 14, 2013 |
| Last update date |
Sep 08, 2014 |
| Contact name |
Yossi Buganim |
| E-mail(s) |
yossib@wi.mit.edu
|
| Organization name |
Whitehead Institute
|
| Lab |
Jaenisch
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE45173 |
Reprogramming by Sall4, Nanog, Esrrb and Lin28 produces high quality iPSCs with a molecular signature of developmental potency that resembles ESCs. |
|
