|
| Status |
Public on Dec 31, 2016 |
| Title |
05_S2_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Forebrain of the finch 60 days post hatch
|
| Organism |
Taeniopygia guttata |
| Characteristics |
tissue: Entire forebrain
male tutor: no
|
| Treatment protocol |
Finches were quickly anesthetized with isoflurane and decapitated. Brains were rinsed with ice cold PBS and the right forebrain was placed on ice in the Trizol solution (Invitrogen).
|
| Growth protocol |
Six zebrafinches were reared with or without male tutor form 30-60 days post hatch (dph).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of ssDNA were hybridized for 16 hr at 45C on GeneChip Zebra Finch Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from zebrafinch 60 days post hatch
|
| Data processing |
The data were analyzed with Robust Multichip analysis (RMA) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Mar 15, 2013 |
| Last update date |
Dec 31, 2016 |
| Contact name |
Kentaro Abe |
| E-mail(s) |
k.abe.789@gmail.com
|
| Organization name |
Kyoto University
|
| Department |
Graduate School of Biostudies, Department of Molecular and Systems Biology
|
| Street address |
Yoshidakonoe, Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16788 |
| Series (1) |
| GSE45208 |
Expression data from Zebrafinch |
|
